1、河北大學碩士學位論文兩屬放線菌的遺傳多樣性分析方法的研究姓名:張曉申請學位級別:碩士專業(yè):微生物學指導教師:張利平20071201AbstractAbstractThisthesisdissertatestheresearchfulcontentandmethodofgeneticdiversityofmicrobesystematacially,andcomparesthefunctionandmeaningofvarietymeth
2、odsinthestudyonmicrobialgeneticdiversityandmolecularidentificationThecontributionofPCR—RFLPanalysisof16S23SrDNAITSandrepPCRetctotheinfraspecifictaxaofactinomycetesandidentificationofinterspecificstrainswasdiscussedbyanal
3、ysisthegeneticdiversityof19urlknownstrainsandthetypestrainsofStreptosporangiumandNocardiopsis,andtheresultswerecomparedwithDNADNAhybridizationThroughstudyingthecompletedsequencesof16SrDNAandDNADNAhybridizationof19unknown
4、strains,theresultsindicatedstrainY70016Y70019belongingtogenusStreptosporangium,andthesestrainscouldbedividedintothreespecies,strainY70017andY70019belongingtothesamenewspeciesthataredifferentfromothertypestrainsofStreptos
5、porangium;strainY70018isalsoanewspeciesofStreptosporangium;strainY70016isthesamespeciesasSterptosporangiumalbumJCM30251Theother15strainsallbelongingtogenusNocardiopsisThroughcomparingofthethirteenstrains,whichbelongingto
6、thegenusNocardiopsiswiththecorrespondingtypestrainsbytheDNA—DNAhybridizationThefinalresultshows:strain10063、20027、20028and20029areallnewspeciesofNocardiopsis;strain20031and20038,20032and20033,20036and20037,20041and20052a
7、rethesamenewspeciesthataredifferentfromothertypestrainsofNocardiopsis;strain20039andNocardiopsisprasinaJCM3336TwerethesamespeciesIntheresearchofTGGE,thebestexperimentalconditionsofTGGEareconcluded,andalltheteststrainsund
8、erthebestconditionswerecarriedoutbythe16S23SrDNAITSPCRTGGEIntheexperimentof16S23SrDNAITSPCRRFLPtwokindsofrestrictionenzymes(Fnu4HIandMnlI)wereselectedtodigestrespectivelythePCRamplified16S一23SrDNAITSofteststrainsTheconcl
9、usionsofexperimentprovedtheproductsofdigestnotonlyhavethesamespecificbandsbutalsohaveintraspecificandinterspecificvarietiesIntheresearchofrepPCRthebestannealingtemperatureofrep—PCRaredifferent,andDNAfingerprintthatwasgai