β-catenin和COX-2在大腸癌中的表達(dá)及意義.pdf

1、中南大學(xué)碩士學(xué)位論文βcatenin和COX2在大腸癌中的表達(dá)及意義姓名：周世權(quán)申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：消化內(nèi)科指導(dǎo)教師：施小六20050501碩士學(xué)位論文英文摘要Theexpressionandclinicalsignificanceof[3—cateninandCOX—2inhumancolorectalcarcinomaABSTRACTObjectiveToinvestigatetheproteinexpressionofp—ca

2、teninandcyclooxygenase一2fCOX21andtheirpossiblerolesinhumancolorectalcarcinomaMethods153archivalspecimensofnormalcolorectalmucosa，adenomaandcarcinomawereanalyzedimmunohistochemically，usinganti—p—cateninandanti—COX一2monocl

3、onalantibody，andastatisticalanalysiswasemployedtoansweqt：Whetllertheirexpressionhaveanycorrelationwitlthepathologicaltype，differentiateddegree，Dukes’stages，infiltrativedepth，lymphnodemetastasisandthree—yearsurvivalofthes

4、eCRCpatients21Arethereanycorrelationamongthedistributionof3catenininCRCcells3Howmuchdidtheectopicexpressionof8。catenincontributetotheoverexpressionofCOX一2incareinomaResults1、a11of15normalmucosaspecimensonlyshowedmembrano

5、us13cateninstainningInl5adenoma，9casesofwhichshowedmembranousB—cateninstairming(60，0％)，and5casesshowedcytoplasmicstaining(333。O％)，only1caseWaSnuclearstainning(6，7％)In123adencarcinomathesenumberswere65(52，8％)，58(472％)and3

6、5(285％)respectivelyInwhicht1elOSSofBcatenininthecytomembrancewassignificantlycorrelatedwiththedifierentiateddegree，lymphnodemetastasisandDtakes’stages儼o05)2)Noneofl5nomlalmucosaspecimensshowedanydetectableCOX2cytoplasmic

7、staining，whileinadenomaandadencarcinomatherewere7of15(467％)and100of123(813％)casesshowedrespectivelystrongintensitycytoplasmicimmunostainingInadencarcinomatheoverexpressionofCOX2wasassociatedsignificantlywiththepathologic

8、altype，Dukes’stages，lymphnodemetastasisandinfiltrativedepth(P005)3)BoththeectopicexpressionofB—cateninandtheoverexpressionofCOX一2hadnocorrelationwiththethreeyearsurvivaloftheCRCpatients4)111eexpressionlevelof13cateninint

9、hecytoplasmawasinverselycorrelatedwiththatinthecytomembrance，andpositivelycorrelated、而ththatinthenucleusbutnoneofthemshowanycorrelationwiththeoverexpressionofCOX2一Conclusion1)ThelossofBcatenininthecytomembrancepronetoinc

10、reaseintheselowdifierentiatedhavinglynnIphnodememstasisandlateDukes’stagesCRCpatients2)TheIevelofCOX一2expressionhaveaconsiderableelevationinthesehavinglymphnodemetastasis，lateDukes’stagesanddeep—infiltratedCRCpatients3)p

11、—catenins，theseresidinginthecytoplasmaandnucleus，maymainlycomefromthedisruptionofAJsinthecytomembrancebutseemtocontributelittletOtheoverexpressionofCOX一2inadencarcinoma4)BcateninandCOX2maybegoodbiomarkersforDukes’staging