白細(xì)胞介素2和白細(xì)胞介素12對人肺腺癌細(xì)胞多藥耐藥逆轉(zhuǎn)的實(shí)驗(yàn)研究.pdf

1、山西醫(yī)科大學(xué)碩士學(xué)位論文白細(xì)胞介素2和白細(xì)胞介素12對人肺腺癌細(xì)胞多藥耐藥逆轉(zhuǎn)的實(shí)驗(yàn)研究姓名：紀(jì)丕軍申請學(xué)位級別：碩士專業(yè)：呼吸內(nèi)科指導(dǎo)教師：劉卓拉2003.6.11m■?！觯憾洞笳艸掌位詩，CExperimentalStudyontheReVersalofMuItidrugResistanceOfHumanLungAdenOcarcinomaCenLinebyInterleukin2andInterleukinl2Abstractob

2、jectiveToinvestigatethereversalefkctofhumanrecombinantinterleukin2(1L2)andinterleukinl2(IL12)onmultidrugresistance(MDR)ofhumanlungadenocarcinomacelllineA549／ADManditspossiblemechanismMethOdsWeinducedhumanlungadenocarcino

3、macelllineA549intomuItidrugresistantcelllineA549／ADMttlmughsl印一wiseselectionwithadriamycin，establishingaMDRcellmodel；evaluatedtheefkctsofthehumanIL2、ILl2andtheircombinationonthemdrlgeneexpressionatlhepmtein1evelwithmonoc

4、lonalantibodybyimmunonowcytometryP—91ycopmteinfunctionwasexaminedaReraccumulationofthenuorescentdmg，adri帥ycinbynowc”omet叫Chemosensitiv“ytoadriamycinwasanalyzedusingtheMTTassayResultsOverexpresionofPgp(thepositiverateofP—

5、gpexpressionwas9215％)wasfoundinA549／ADMcells，whiletheP—gpcouidonlybedetectedin(25土05)％ofA549cells，thedifferencewassigni矗cant(PO01)1L2、lLl2andtheircombinationcouiddownregulateP—gpexpression，enhancctheintracellularaccumula

6、tionofadriamycin，andimproVethechemosensitivityofadriamycin，moreoverthecombinationofIL2andILl2hadadditiveefkctThisc”okine—inducedreVersalofMDRwasscronglytjmedependent，withmaximalef弛ctsaner48and72hofcytokinetreatmentConclu