喉癌組織中PTEN基因蛋白表達(dá)的初步研究.pdf

1、青島大學(xué)碩士學(xué)位論文喉癌組織中PTEN基因蛋白表達(dá)的初步研究姓名：劉付星申請學(xué)位級別：碩士專業(yè)：耳鼻咽喉科指導(dǎo)教師：趙書佑2003.4.8STUDYoNEXPRESSIONLARYNGEALCAOFPTENGENEPROTEININRClNoMASTlSSUEAbstract【Objective】PTENgeneisasuppressorgenethatdephospgorylatesphosphatidyrlinositol34，5t

2、riphosphate，anintermediateintheP13一K／AktsignalingpathwayItisknowntobeassociatedwithtumourdevelopmentinseveralhumancarcinomasStudiesonPTENhaveimprovedsince1997PTENisatumorsuppressorwhichhassequencehomologywithdualspeefici

3、t／phosphataseItisamultifunctionalproteinendowedwithaphosphataseactivitycapableofdephosphorylatingbothtyrosine，serine／threoninephosphateresiduesonproteinandphospholipidsofthephosphatidyUnositoIpathwayPTENappearstobemutate

4、datconsiderablefrequencyinseveraltypesofhumantumors，includingthosefrombrain，breastendometrium，andprostatePTENplayanimportantroleinpathogenesisoftumor，tumorcellinvasionandmetastasisInthisreview，wewillinvestigatetheexpress

5、ionoftumorsuppressorgenePTENproteintofindtheassocicationbetweenproteinexpressionofPTENandclinicalpathologicaldifferentiation，stageandlymphnodesstatusoflaryngealcarcinomas【Methods】UsingSP，westudied50laryngealsquamouscellc

6、arcinomasspecimensand40vocalcordpolypswithmousemonoclonalantibodysofPTENDiseasestagesweredeterminedusingthepTNMclassificationaccordingtoUICCcriteria(1992)AllspecimenswereapprovedbypathologicalexaminationafteroperationAll

7、datawereanalyzedbyExcelstatisticsoftware[Results]WefoundthattumorsuppressorgenePTENwasexpressedlowlyinlaryngealsquamouscellcarcinomas(Theratewas62％1Buttheexpressratewas100％incontrastThedifferencesweresignificantstatistic

8、aIly(p001)PTENwasexpressedinhighly，moderately，poorlydifferentiatedsquamouscellcarcinomasatfrequenciesof824，571，20％respectivelyThedifferencesweresignificantstatistically(p00s)Similarly，wefoundtherewererelativitybetweenthe