雞tfam遺傳多態(tài)及其與ATPase6基因表達(dá)相關(guān)關(guān)系的研究.pdf

1、中國農(nóng)業(yè)大學(xué)碩士學(xué)位論文雞tfam遺傳多態(tài)及其與ATPase6基因表達(dá)相關(guān)關(guān)系的研究姓名：初蔚琳申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：動(dòng)物遺傳育種與繁殖指導(dǎo)教師：趙興波20070501中囝農(nóng)業(yè)大學(xué)碩￡學(xué)位論文摘要AbstractMitochondrionisallimportantandspecial唧ellein蝴oticcell95％ofthewholeATPproducedbyhumancellisproducedinmitochondrion

2、Accordingtothesefact，themitochondrioniscalled“energyfactory“AsamemberofTypicalHMGfamily，tramisaproteincodedbynucleargeneandithasthefunctiontocombinewithDN丸T細(xì)misthemainconductorofthenumberofmtDNAcopiesOtherwise，iti暑aimpor

3、tantelemontthatCallmaintainthestabilityofmtDNAsinceitcanformthenuclenrpackconfigurationInthisstudy，WeconductadiscussionoftheinfluenceofmitochondriontransferfactorAanditsgonedifferenceonthechickenmetabolizerelatedcharacte

4、rs，bythehelpofresearchonmitochondriontransferfactorAandothergenerelatedofexperimentalchickengroupswithdifferentgrowthrateWethenforecasttherelationshipbetweenmitochondriontransferfactorAandchicken’sgrowthcapacity，soastogi

5、vestrongevidencefortheimprovementofanimalimportanteennomicalfactorsAccordingtothesequencingresultofRTPCRWefoundthreeSNP(C327T，C427—A，G660A)inCDSsegmentofchickonmitochondriontransferfactorAgoneandfourSNPin3’UTRregionofgas

6、geneaccordingtotheanalysisofrealtimePCRdata，weobservedthat咖mgeneandATPase6sexpressionofthecockgroupandhengrouphavenOnotabledifferenceeventhroulghthereexsistsobviousdiffcrcnccbtwcenthesetwogroupsmoreoverwehavegiVOnmoreevi

7、dencetothisconclusionbythehelpofrelativityanalysehowever，wefoundthatgamandmRNAlevelofATPase6havecloserelationthroughrelafivityanalyse，sowehaveprovedtheconclusionthatthemitochondriontransferfactorAcabconductthetransferofm