Fas-FasL基因與低劑量化療聯(lián)合中藥抗腫瘤減毒增效機理的研究.pdf

1、中日友好臨床醫(yī)學(xué)研究所碩士學(xué)位論文FasFasL基因與低劑量化療聯(lián)合中藥抗腫瘤減毒增效機理的研究姓名：牛晉衛(wèi)申請學(xué)位級別：碩士專業(yè)：外科學(xué)（普外科）指導(dǎo)教師：黃林平20090101TheStudyofMechanismofCombinedAnti—tumorTherapyofFas／FasLGeneandTCMandLowDoseChemotherapyforLowerToxicityandBetterOutcomeAbstractob

2、ject：ClinicallyspeakingsurgerychemotherapyradiotherapyTCMtherapyandimmunotherapyarethemaintherapeutictoolsforhepatomaamongwhichchemotherapyplaysthemostcriticalroleintheadjunctivetherapyButthechemotherapyunavoidablyharmed

3、thebody’SnormalfunctionresultinginleucocytedecreaseanddescendingofimmunefunctionTheaimofourresearchistolowerthedoseofchemicaldrugs，withthestrengtheningbodyresistancefunctionoftraditionalChinesemedicinetotreatlivercarcino

4、maeell，andalSOtoapproachthemechanismoftheaccomplishmentoflowerpoisoningandbetteroutcomeusingFas／FasLgeneasthecutinpointMethods：ToobservetheeffectofcombinedtreatmentoflowdosechemicaldrugandTCMonhepatomathroughtheeocultiva

5、tionoflivercarcinomacelltreatedwithaseriesoflowdoesofchemicaldrugsandlymphocytetreatedwimTCMToelucidatethemechanismthroughthedeterminationofexpressionofFas／FasLgeneofthechemicaldrugtreatedlivercarcinomacellandthroughtheo

6、bservationofthecocultivatedeffectofthetwotypesofcellswhichhavebeenintervenedbyFasLmonoclonalantibodyResults：1Thegrowthoflivercarcinomacellwasbarelyinfluencedbylowdosechemicaldrugsbutlivercarcinomacellstreatedwithlowdosec

7、hemicaldrugsrevealedhighsensitivitytothelymphocytesandtendedtotakeonapoptosisprogresswhenlymphocyteexists2TheabilityofthelymphocytetokilllivercellcarcinomaWasenhancedafterbeingtreatedwithTCM3Thelymphocytetolivercarcinoma

8、celleffectmainlydependsonthewalltowalltouchofthecells4EL7402iStoexpressFasandFasL5TheexpressionofFasofBEL7402intervenedbydifferentconcentrationsofpharmorubicinhasnodifferencecomparedwimthecontrolgroup6BEL7402intervenedby

9、lowdoseofpharmorubicincallreducetheexpressionofFasL7BEL7402intervenedbycurativedoseofpharmorubicinhasahigherexpressionthanthecontrolgroup，althoughthereisnostatisticaldifference9Lymphocytekillsthelivercarcinomacellthrough

10、theinteractionbetweenFasandFasLConclusion：ThecombinedapplicationoflOWdoseehemicaldrugsandtonicTCMcanreducethetoxicityofchemicaldrugs，protectlymphocyte，enhancelymphocyteimmunitySOastostrengthentheabilitytokilllivercarcino