Ri質(zhì)粒轉(zhuǎn)化三倍體毛白楊及外源基因表達(dá)的研究.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文Ri質(zhì)粒轉(zhuǎn)化三倍體毛白楊及外源基因表達(dá)的研究姓名：劉興菊申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：林木遺傳育種指導(dǎo)教師：周懷軍楊敏生20050612StudyOHRiPiasmidTansformationandExogenousGeneExpressionofTriploidChineseWhitePoplar(Populustomentosa)Author：LiuXingiuSpeciality：Forestgeneticsa

2、ndtreebreedingAdvisor：ZhouHuaoun，YangMinshengAbstractInthisstudythreeAgrobacteriumrhizogenesbacterialstrains30148，15834andZALFinfectedtheleafexplantsoftriploidChinesewhitepoplarthehairyrootswereobtainedfromleafexpIantsHa

3、iryrootinvitroculturesystemwasestablished，hairyrootregenerationplantwasinducedatcontainingdifferenthormonecultureTransformedplantoftriploidChinesewhitepoplarwasconfirmedbytheshapecharacteristic，agropineanalysis，PCRanalys

4、isIndicatedthatTDNAofRiplasmidhadalreadybeencombinedintointheregenerationplant91％oftransgenicplantofthetriploidChinesewhitepoplarleafshowedwinklesymptomcomparedwiththecontrast，theothersgrewnormallyTheleavesoftransplantha

5、dthecharacteristicofthespontaneousrootinMSomediumTherateofrooting，lengthofroots，numberofroots，heightofplantandphenotypicvariationoftransgenictriploidChinesewhitepoplarclonewerestudied，Resultsshowedthat：rateofrootingoftra

6、nsgenictriploidChinesewhitepoplarwaSincreasedsignificantlyandhadextensiverOOtsystemcomparedwiththecontrast，thenumberofrootof78％transplantclonewasincreasedcomparedwiththecontrastandthelateralrootsformedaclusteranddevelope

7、dwell，abilityofrootingWasincreasedsignificantly；Theseedlingsoftransplanthadaremarkabledifferenceinleafshape，leafarea，petiolelengthandStemlengthcomparedwiththecontrast，majorityshowedthatleafwithered，long／wideincreased，lea

8、fareadecreased，petiolelentgthreducedandgrowthofhi曲decreasedThecloneswereselectedsuchas307，328，363whichgrewnormallyandhadlittlevariationingrowthAbout30differenttransgeniccloneswereselectedfromArhizogenes30148triploidChine

9、sewhitepoplartransplantandchlorophyllcontentandphotosynthesizerateoffunctionofleavesweremeasuredResultsshowedasbelow：contentofchlorophyllhadsignificantdifferenceamongdifferenttransgenicclone，onlytwotransgeniccloneshadlow

10、contentofchlorophyllb，othertransgenicclonescontentofchlorophylla，chlorophyl[bhadalinearcorrelationwithchlorophyll(ab)；Photosynthesizerateof85％transgenicclonesworelowerthanthecontrast，contentofchlorophylloftransgenicclone