甘草愈傷組織誘導(dǎo)及體細(xì)胞胚的發(fā)生.pdf

1、河北農(nóng)業(yè)大學(xué)碩士學(xué)位論文甘草愈傷組織誘導(dǎo)及體細(xì)胞胚的發(fā)生姓名：霍云謙申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：劉桂茹楊學(xué)舉20050620CallusinitiationandsomaticembryogenesisofG／y穢橢豇口uralensisfischAuthor：HuoYunqianMajor：CropGeneticsandBreedingAdvisior：ProfessorLiuGuiruProfessorYangXu

2、ejuAbstractThe1icorice(Glycyrrhizallrtllensisfisch)，whoserootsandrhizomesaremostcommonChineseherbalmedicineiSakindofperennialSOffurticosaherbalplantsfromGlycyrrhizaspeciesLegumefamilyBesidesmedicalvaluethelicoriceiSwidel

3、yusedinfoOd，beverage，cigarette，cosmeticsandotherindustriesasadditiveAsnaturalresourcescan’tfulfillrequireofmedicineandotherjndustriesCultivalionandtissuecultureareconsideredtobehopefulwaytoalleviatethecrisisofnaturalreso

4、urcesTissueculturetechnicisagoodmethod，itcancontrolgrowconditionofplants，acceleratepaceofpropagate，anddistilleffectualelementfromcellsGlycyrrhizauralensisFischasmateria】inthetestButforlicoricetissueculturetechnologywasno

5、tmatureonlyfeWpaperswerereported，thereareneportsaboutSomaticembryogenesisandeffectofhormonetocallusWestudythecourseofcallus、effectofhormonetocallus、suspensionculture，observethecourseofSomaticembryothroughespialofcytology

6、，intenttosetupsystemofinducecallusDefinedthecallus’slatetotranslateSomaticembryoOffcrtheoryandtechnicfoundworkofpropagationanddistijIeffectuaielementfromcells7fherusu】tasf01lOW“1Explentbegantoappearcallusafter3days，theca

7、llusOCCUrfrommorphologyicextremityextendtothewholeexpeIntalongaxis2StudytheeffectofhormoneandtheircollaborationtoinducecallusCalluscan’tbeinducedonMSculturemediumwithsinglehormonethebestcombinationofinducecallusisO2mg／LN

8、AA10me礦tBA10mI∥L24一D3StudytheeffectofhormoneandtheircombinationtOcalluscontinueculturewhenthecalluswasculturedonMSculturemediumwith6一BA10～20m2／LandlowerconcentrationofZT、KTthenembryogeniccallusappearedWeobtainedcallustos

9、uspensioncultureandbrownandgreen，granuleembryogeniccallus4Studytheeffectofsuspensionculturethroughhormoneandadditionalmatterestablishtissueculture’Ssuspensionsystem：MSculturemediumwith02mg／LNAAO1mg／LBAO1mg／LKTGetSomatice

10、mbryointhesuspensionsystem5Insuspensionculture，cellgrowcurveshowS’，Thetimeoflogarithimgrowthappearsafterinoculation1020days；Masswastageofsucroseinlogarithimgrowth；MasswastageofNH4infrontandbehindlogarithimgrowth，pHandEle