利用MGE-PCR、SSR-PCR和RAPD-PCR技術(shù)研究錐蟲亞屬種間的遺傳變異與進化關(guān)系.pdf

1、中山大學(xué)碩士學(xué)位論文利用MGEPCR、SSRPCR和RAPDPCR技術(shù)研究錐蟲亞屬種間的遺傳變異與進化關(guān)系姓名：鄭嘉瑜申請學(xué)位級別：碩士專業(yè)：動物學(xué)指導(dǎo)教師：倫照榮20050609!坐盔生堡主蘭堡笙苧——一————』!!旦一AbstractMobilegeneticelement(MGE)一PcRsimplesequencerepeat(ssR)一PCRandrandomamplifiedpolymorphicDNA(RAPD)一PCR

2、techniqueswereusedtoanalyzethegeneticvariationandtheevolutionrelationshipsamongthreeTrypanosomaequiperdumstrains(oneisolatedfromChina，onefromAfrica，andthelocalityoftheotheronewasunknown)，tenZevans／strains(7fromAsia／china

3、，2fromSouthAmericaandonefromAfrica)andfive工bruceistrainsfr∞AfricaAlltbxeemethodscaizproduceanobviouspolymorphismintheeighteentrypanosamestrainsWithacons／derationofthedatageneratedfromthethreetechniques，allZovansistainsan

4、dtwoZequiperdumstrainsweregroupedintoonebranche，andallZbruceistrainsandoneZequiperdumweregroupedintoanotherbranceinthedendrogramwhichwasconstructedwithUPGMAsoftwareZbruce／showedahighestintraspeciesgeneticvariabilitywitha

5、similaritycoefficient(鰳of5121％，whereasZevansiandZequiperdumstrains，was7776％and6213％respectivelyBycomparisonoftheScbetweenTegBflSl“strainsisolatedfromChinaandSouthAmerica，higher(81。2娟)SCwasobserved，whichwashigherthanthose

6、betweenthestrainsfromChinaandAfricaandstrainsbetweenSouth^lnericaandAfrica(6346糯and7005％)respectivelyThisresultfuthersupportsthehypothesiSthatZevansistrainsfromChinaandSouthAmericamayhavearisenfromasingle1ineageFurthermo

7、re：theevolutionrelationshipsamongZbruceLZevans／andZequiperdumwerealsodiscussedinthisthesisInadd／t／on，likeRAPDmethod，ourworkalSOindicatedthatMGEandSSRarepowerfulmethodsforanalysiSofgeneticvariationamongTrypanozoonspeciesK