P63、K3在人角膜緣組織中的表達及意義.pdf

1、y9500S1分類號：R772學(xué)校代碼：學(xué)號：福建醫(yī)科大學(xué)碩士研究生畢業(yè)論文P6；3、K3在人角膜緣組織中的表達及意義103q22003230TheStudyofPti3、K3ExpressionandSignificanceinHum￡IrlLimbus所在皖(系)：第二臨床醫(yī)學(xué)院研究生：張丹鳳學(xué)科(專業(yè))：眼科畢導(dǎo)師：莊銘忠主任醫(yī)師、教授研究起止日期：2004年11月至2005年l2月二零霉卉年三月ThestudyofP63、K3e

2、xpressionandsignificanceinhuman1imbusPostgraduate：ZhangdanfengSupervisor：ProfessorZhuangmingzhongAbstractPurposeTodetectP63、K3expressioninhuman1imbus，toapproachthepossibilityofP63actedasthemarkerofhuman1imbalstemcellsMet

3、hod8freshnormalhumancornealtissueswereobtainedfrom8donorswhosufferedseriousruptureofglobeweobtainedcornealtissuesthroughdoingeviscerationofeye24cornealtissueswereobtainedfrom24donorswhowerediagnosedlimbalstemcelldeficien

4、cy(LSCD)，ThediagnosisofLSCDisbasedmainlyonclinicalsignsweobtainedthesecornealtissuesthroughdoingeviscerationofeyeaftersurgeryThetissuespecimenswereinⅪnediatelyfixedin10％phosphatebufferedformalinfor1day，andembeddedwithpar

5、affinParaffinsections(4脅thick)werethencutforinImmunohistochemicalStainingexperimentsResultsp63proteinwasimmunodetectedonlyinthenucleioflimbalepithelialbasallayer，butnotinmost1imbalsuprabasal，paracornealandcornealepitheli

6、alcellsincornealsoflocal1imbalstemcellsdeficiency，becausethereareprobablysomehealthlimbalstemcellsinlimbus，p63proteinwasimmunodetectedonlyinthenucleioflimbalepithelialbasallayer，buttheexpressionisreducedincornealsofdiffu

7、selimbalstemcellsdeficiency，withtheextensivelossoflimbalstemcells，p63proteinwasnotimmunodetectedinwholecornealK3proteinwasnotimmunodetectedonlyinthenucleiof1imbalepithelialbasallayer，butin1imbalsuprabasal，paracornealandc