輻射誘導(dǎo)pEgr-p16的表達(dá)及抑瘤作用的實(shí)驗(yàn)研究.pdf

1、中國人民解放軍軍事醫(yī)學(xué)科學(xué)院放射醫(yī)學(xué)研究所博士學(xué)位論文輻射誘導(dǎo)pEgrp16的表達(dá)及抑瘤作用的實(shí)驗(yàn)研究姓名：劉建香申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：放射醫(yī)學(xué)指導(dǎo)教師：蘇旭20040528ABSTRACTExperimentalstudyonexpressionofpEgrp16andtheroleofanti—tumorinducedbyionizingradiationInthisstudyaccordingtothemechanismthat

2、ionizingradiationcanactivateearlygrowthresponse1(Egr1)genepromoter，whichinducestheexpressionofdownstreamgenesandantitumoreffectofatumorsuppressorgenep16，thus，arecombinantplasmidwhichcontaininghumansuppressorgenepl6andrad

3、iosensitivepromoter(Egr一1)wasconstructedIthasbeendemonstratedthat60Coyraysirradiationcanenhancetheexpressionofp16inHeLaandSMMC7721cellswhichweretransfoctedbyrecombinedplasmidspEgrp16invitronwashigherthanthatinHeLaandSMMC

4、7721ceilswhichwerenottransfectedTheresearchalsodemonstratedthatradiationcandecreasetheexpressionofCDK4andCyclinD1proteinlevelanddecreasetheexpressionofc—mycgenetranscriptionallevelinHeLaceilsandSMMC一7721cellswhichweretra

5、nsfectedbyrecombinedplasmidspEgrp16Theincreaseordecreaseofexpressionof916andcorrelativedownstreamgenesdependsonEgr1promoterthatactivatedbyionizingradiationTheabovementionedrolesandradiationeffectsinducedG2／Marrestandinhi

6、bitedeellgrowthinHeLaandSMMC一7721cellsfinallyThisresearchhasanimportantandpracticalsignificanceforinvestigatingnewtherapymethodthatincreaseradiotherapeuticeffectsonmalignanttumor【KeyWords】p16gene，Egrlpromoter，pEgvpl6reco