MMP-2、MMP-9及TIMP-3在膀胱移行細胞癌中的表達及臨床意義.pdf

1、山西醫(yī)科大學碩士學位論文MMP2、MMP9及TIMP3在膀胱移行細胞癌中的表達及臨床意義姓名：邢濤申請學位級別：碩士專業(yè)：泌尿外科指導教師：劉紅耀20100405山西醫(yī)科大學碩士學位論文ExpressionsofMMP一2，MMP一9andTIMP一3inhumantransitionaIcencarcinomaofbladderandtheirclinicalsignilicanceAbstractBackgroundandpourp

2、ose：1ToiIlVestigateⅡleeXpressionofmatrixmetalloprotei徹se2，9(MMP2，MMP一9)aIldtissueinhjbitorofmetalloproteiIl乏齡3(TIMP3)in昀nsitionalcellcarCinomaofbladdera：bout也eexpressionintens毋鋤ddistribution2Tbdetecta：berr鋤tpromotermemyl

3、ationofmegenebytissuei11：hibitorofmetaIloprotein舔e3(TIMP3)鋤dt0disc潞sthe缸lctionofTIMP一3強ditSclinicalsigIlific鋤ceintIunorMethods：1UsingamethodofinununohistoChemicalsta珧aIldalli曲一reS0lutioncolor鯽llicallalysisofmedicalsystem

4、s，theexpressio璐ofMMP一2，MMP9aIldTIMP3werea舳lyzcdinh眥鋤s鋤plesfrom38casesofbladdercarcinoma(tuInor印up)，15c邪esofno鋤albladde“controlgroup)2TIMP3genepromotermethylationw嬲(1etectedbymethylation—specificPCR(MSP)inhumansamplesfIro

5、m38caSesofbladdercarcinoma(tumorgroup)，15caSesofnomalbladde“control掣oup)Result：1MMP2，MMP一9，TIMP3、Ⅳ嬲si弘ifica11_tlyhi曲eriIlBTCCtll鋤證adjacentnonnalbladdermucosa(PO01)nomalbladderepithelialMMP2，MMP9Ⅵ慣enegatiVeexpressio瑪TIMP一

6、3expressionislow2MMP2，MMP9eXpressioniIltumor印ithelialcellsandexpressionofnor卜uIlifomlimensi夠distIibutied，謝thmeserlchyInmtissue，bloodVesSelsneartlle馴【rfke鋤dde印tissuesofbladdertu】norinv硒ionintotlleleadingedgeofpositivecell

7、sexpressas缸Dngintcnsi夠TIMP3expressioniIlt哪orcellsaIldexpressionofrclatiVelyintellsit)rdistributionismlifo冊BladdernonIlaltissuesandbladdercallcertissueMMP一2，MMP9andTIMP一3opticaldens時詞ueofthedi虢rencewaSsigIlific觚t3MMP一2a11

8、dMMP9werehighlyexpressedintumors鋤plesaIldtheirexpressionmteswereincreaSed誦m莎adesaIldstagesTIMP3w弱alsohighlyexpressedintumors鋤plesanditsexpfessionrateswaSincre弱ed麗m伊adestoobutnot研tllstages4Only5out38(132％)c嬲eshadTlMP一3met