36672.黑腹果蠅種組cu，znsod和mnsod基因的進化和分子系統(tǒng)學研究

1、湖北大學碩士學位論文黑腹果蠅種組Cu，ZnSOD和MnSOD基因的進化和分子系統(tǒng)學研究姓名：李志新申請學位級別：碩士專業(yè)：遺傳學指導教師：曾慶韜20080601AbstractDrosophilamelanogasterspeciesgroupsubjectstoGenusDrosophila，SubgenusSophophora，includingmorethan180species，mostofwhichhavebeenclassi

2、fiedintol2subgroupsbasedonmorphologicc：hafactersSeveralstudieshavebeendonebasedonmorphology，biogeographyandmolecularbiologycharactersbyfar，butitstaxonomyandphylogenyremainscontroversial，duetothelackofknowledgeofthephylog

3、eneticrelationshipsofthespeciesByPCRandclonesequencingmethodsweacquired20nucleotidesequencesofCu，ZnSODgeneand24nucleotidesequencesofMnSODgeneof，”e腸，lDg口s紀，speciesgroupMoleculerevolutionresultsshowthecodingregionsofthetwo

4、genesarehighlyconservedTheevidencesfromhi曲ratiosofKs／KaandGCIIIcontentsshowthatthecodingregionsundergoespurificationselectionDeletion／insertionsegmentsareallobservedintheintronsofthetwogenes，thisregionincludesabundantinf

5、orrnationsofthephylogeneticrelationshipsofthespeciesDrosophilapseudoobscurawasselectedastheoutgroup，phylogenetictreesarcconstructedbasedonthesequencedataofCu，ZnSODandMnSODgeneswithNeighbourjoining(NI)，Maximumlikelihood(M

6、L)andBayesianmethodSupportingvalueswerecalculatedbybootstrapTheresultshowsthatmelanogasterspeciesgroupclusteredinthreemainlineages：(1)ananassaesubgroup；(2)montiumsubgroup；0)melangastersuzukii—elegansficusphila—eugracilis

7、takahashiiTherelationshipsbetweenthemelangastersuzukiielegansficusphila—eugracilistakahas—IIl豇lineagesareunclear；inthe掰e腸聹肛s把，subgroup，DsimulansandDsechelliaarcthemostcloselyrelatedspeciesKeywords：phylogeny；melanogasters