黃牛體細(xì)胞-家兔卵母細(xì)胞種間核移植研究.pdf

1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文黃牛體細(xì)胞家兔卵母細(xì)胞種間核移植研究姓名：劉亞申請學(xué)位級別：碩士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：章孝榮20020101StudyonsomaticnucleartransferininterspeciesusingbovineearfibroblastasdonorandrabbitoocyteasrecipientPostgraduate：LiuYaTutor：profZhangXiaorougandasso

2、ciateprofHeMeide(Cortegeofanimalhusbandryandaquiculture，AnhuiAgriculturalUniversityHefeiChina，230036)AbstractTheexperimentsofsomaticnucleartransferwereconductedbyuseofbovineearfibroblastasdonorandrabbitoocyteasrecipientT

3、heresultsshowedthat202reconstructedeggswereobtainedbythefusionoffivekindsofbovineearfibroblasts(freshorrefrigeratednon—Gocells，freshorrefrigeratedcellsat100％cofluencyandrefrigeratedserumstarvedcells)and436rabbitoocytes，t

4、hefusionratewas463％，andin180reconstructedembryosculturedinvitro，112ofthemdevelopedto2cell(6212％)，26developedtomorula(144％)，20developedtoblastocyst(111％)，whichsuggestingthatbovinesomaticcellscouldbededifferentiatedinrabbi

5、tOocytes，andreconstructedembryosininterspeciescoulddeveloptohatchedblastocystThefindingsofthisresearchalsoindicatedthatbovineearfibroblastspreservedat4℃within19dorfrozenat——196。Chadthenormalproliferatingandwall—attaching

6、abilitywhentheywerere，cultured；thecellsrefrigeratedat4℃for14dcouldstillserveasdonor；mortalityrateofreconstructedeggswereincreasedsignificantlywhenaddingcytochalasin(cB)intofusionsolution(PO05)，butthemortalityrateofeggsre

7、constructedwithmultiparousrabbitOocyteswassign!ficantlyhigherthanthosewithyoungones(P001)；theembryosreconstructedwithserthn—starvedcellsweresignificanthigherintherateof8～16一cellormorulastage(P005)，andblastocyststagefP001