利用RAPD標(biāo)記進(jìn)行黃瓜遺傳多樣性評(píng)價(jià)及黃瓜核心種質(zhì)構(gòu)建初探.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文利用RAPD標(biāo)記進(jìn)行黃瓜遺傳多樣性評(píng)價(jià)及黃瓜核心種質(zhì)構(gòu)建初探姓名：張廣平申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：向長(zhǎng)萍李錫香2002.5.1AbstractGeneticbreeding，whichbasedongeneticdiversityofgermplasm，willbeimprovedbyestablishingcorecollectionamethodtomakingmoreefficientuseo

2、fpresentgermplasmRandomamplifiedpolymorphicDNA(RAPD)，anewlydevelopedmolecularmarkertechniques，isapowerfulmethodforstudyingbio—diversityandestablishingcorecollectionIthasbeenpopularizedforitsrapiditysimplicityandrevealing

3、thegeneticdiversityonDNAlevelTwopartswereincludedinthepaperFirst，thegeneticdiversityof90cucumberaccessionswasdetectedbyRAPDanalysisSecondlythemethodofestablishingcorecollectionbyRAPDdataWaSresearchedResultsofcucumbergene

4、ticdiversityanalysisshowed：1Twohundredsprimerswereusedtoscreenthe90individualcucmnbergenotypes，and25outofthemhavehighlypolymorphicThesimilarcoefficenceof90cucumbergenotypeseachotherwereestimatedwitllthebandsofRAPDproduct

5、ionandclusteredtheminto3clusters：European&Americanhuananxingandhuabeixing2SimilarcoefficenceamongaccessionswerenolessthanO59，implyingcucumberisanarrow—basedgermplasmAlthougheachaccessionshadauniquemarkerbands，indicatingt

6、hatRAPDanalysisWasusefulincucumbergenotypicdifferentiation3Sevenprimers($470、S513、S25、$340、$27、$503and$29ie、werechosenfromtwenty—fiveprimerstoestablishtheRAPDfingerprintofcucumberThatis4CucumissatiVUSLVarXishuangbannanes

7、is“etYuanamutationofcucumberwasclusteredtogethertoaspecialgroupinthestudywhichimplieditsspecialstatusincucumbergermplasmTheresearchonestablishingcorecollectionshowed：1ixcorecollectionswereindividuallyestablishedwithdiffe

8、rentsamplesizeof10％、15％、20％、25％、35％and45％TheefficiencyoftheindividualcollectionsWascomparedwithnumberofpolymorphicloci，numberoflacklociandgeneticdiversityindexTheresultsshowedthat25％istheidealpercentagetocucumbercorecoll

9、ection2Threestrategies：randomsample，maximaldeflection，maximalgeneticdistancewerecomparedfordevelopingcorecollectionwithnumberofpolymorphicloci，numberoflacklociandgeneticdiversityindexAndtheresultdemonstrateds仃ategyofmaxi