桃品種遺傳多樣性分析及核心種質(zhì)的構(gòu)建.pdf

1、中國農(nóng)業(yè)大學碩士學位論文桃品種遺傳多樣性分析及核心種質(zhì)的構(gòu)建姓名：李銀霞申請學位級別：碩士專業(yè)：果樹學指導教師：李天紅20060601AbstractTheoptimalsamplingstrategyofprimarycorecollectionofpeach(尸r“nuspersica(L)Batsch)fromtheentirecultivarcollectionofNationalPeachGermplasmResourcesR

2、epositoryinBeijingwasstudiedtoestablishtheprimariycoreeollectonofpeach，using558accegsiouslistedinthefruitgermplasmresourcescatalogueand18morphologicandagronomictraitsasbasicdataSSRreactionsystemwasoptimizedonpeach，andgen

3、eticdiversitywasanalysisedusingSSRmolecularmarkerandmorphologicalmarkerinthepeachprimarycorecollectionSamplingschemewasestablishedandconstructedthecorecollectionofpeacheultivars，atthesametime，thevalidityofthecorecollecti

4、onwasdetectedTheresultsw日easfollowing“l(fā)Theappropriateentiresamplingratiowasl0％forprimarycorecollectionofpeachUnder10％entiresamplingratio，theoptimalsamplingschemewasgroupingprinciplebasedonvarietygroupcombiningwithsamplin

5、gproportionwithingroupbasedonproportionofgeneticdiversity(G)whenrandomsamplingmethodappliedwithingroupUsingthissamplingstrategytoacquiretheprimarycorecollectionof56accessionsfrom558accessionsofbasecollectionofpeachcultiv

6、am，andtheprimarycorecollectioncouldrepresentthegeneticdiversitiesoftheentirecultivarcollection2‘Shinokubo’peach(Prunuspersiea(L)Batsch)wasusedasexperimentalmaterialSSRPCRamplificationsystemwasimprovedonpeachusingorthogon

7、aldesigndirectanalysisandcompletelyrandomizeddesignoftwofactors30cultivarswereusedtetesttheSSRsystemTheresultsshowedthattheoptimalconcentrationofMgzwas15N20mmol／L；thedNTPwas020028rnmoVL；theprimerwasO206pmol／L；DNAwas50100

8、ng；TaqDNApolymerasewas1Uin25pLreactionsystemThissystemwassteadyandreproducible3，ThegeneticdiversityofprimarycorucollectionofpeachwasstudiedwithSSRmolecularmarkerTotally22polymorphicspecialprimerswerescreenedoutand116alle

9、lesweredetected，averagenumberwas53allelesineachlocnsTheorderofgeneticdiversityofeachvarietygroupwasSweetpeachCrisppeachFlatpeachNectarineHoneypeachYellowfleshedPeachThegeneticdiversityofChinesecultivarswerehigherthanintr

10、oducedcultivars’Theclusterresultshowedthatallthematerialwe∞taxonomiedinto5typesat0786similaritycoefficientandvarietygroupswerenotdifferentiatedandintercrossedamongthem4Theparameters，suchasindexofgeneticdiversityratioofre

11、tainingandratiooflosing，werestudiedindifferentsamplingratiooffoursamplingmethodusingnmrphoagronomicdataandallelicdataofprimarycorecollectionThecorecollectionwasevaluatedbysomequantitivecharactersTheresultsshowedthatclust

12、ersamplingwasbetterthanrandomsampling，andclustersamplingofSSRMORdatawasthebestinallsamplingmethodsatthe80％ofsmnplingratio，including45accessionsAtlastthecorecollectionofpeachretained966％allelesofprimary螄collectionand9949％

13、charactersofphenotypeofinitialcollectionTheCOlecollectioncouldrepresentthegeneticvarianceoftheinitialgermplasmKeywords：Peach(Prunuspersica(L)Batsch)，SanaplingstrategySSR(SimpleSequenceRepeat)，GeneticdiversityCorecollecti