小麥品種Glu-B3位點(diǎn)LMW-GS基因的檢測(cè)和序列分析.pdf

1、安徽農(nóng)業(yè)大學(xué)碩士學(xué)位論文小麥品種GluB3位點(diǎn)LMWGS基因的檢測(cè)和序列分析姓名：武曉蓓申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：馬傳喜2011AbstractThe285Chinesewheatlandraceshadbeenscreenedusing10allelespecificPCRmarkersatGluB3locusGlu—B3allelesof284varietieshadbeenidentifiedand，1vari

2、etyisallunknowalleleOfthese，thenumberofcalleleismorethanothertypes，andmakesup5649％eandiallelesarethesecondwhichcontain3228％Thea、b、d、f、gallelestakeup1860％、2737％、2877％、1298％、1579％，respectivelyHowever，thehalleleistheleastof

3、355％andtheunknowalleleisonlyO36Theorderofnineallelesisci=edbagphAnewhaploidtypeofGluB3—3werefoundinthe114thVariety(shijiazhuang54)whenwedetectedalleleiAndthen，accordingtoGlu—B3eachallelesdistribution，weselectonlycontains

4、oneallelegenetodetectfull—lengthofgeneAnd，full—lengthsequenceofthealleledgenewasgotTheballelewhichhaslargereffectintheglutenstrengthaccountsforthenumberof2737％howevertheCalleletwiceofballeleaccountsfor5649％andhasthesmall

5、esteffectinChinesewheatlandracesForRmaxCalleleismostwidelydistributedbutcontributesthesmallestToLMWGSofallelescompositiontofindtheoptimalcombinationvarietiesandimportqualityvarietiescouldbeaimportantwayimprovingourwheatq