荷斯坦奶牛Leptin和ESR基因多態(tài)性與繁殖力相關(guān)分析研究.pdf

1、寧夏大學碩士學位論文荷斯坦奶牛Leptin和ESR基因多態(tài)性與繁殖力相關(guān)分析研究姓名：李鵬申請學位級別：碩士專業(yè)：動物遺傳育種與繁殖指導教師：史遠剛20100501AbstractTheFertilitytraits’data、衍也completeDHIrecordsfromfivelargescaledairyherdswerecollectedtoanalyzetheirgeneticparametersbyuseofMTDFREM

2、LsoftwareinthepresentstudyMoreover，PCR—SSCPtechnologyWel“employedtoanalyzethecorrelationbetweenLeptingene，ESRagene’Spolymorphismandfertilitytraitsin94HolsteinCOWS111eresultsshowasfollows：l、Heritabilityofstartmatinginterv

3、altraits，thefirstandthesecondparityintervaltraits，thesecondandthethirdparityintervaltraitswere02，001and003，respectively；Nevertheless，geneticcorrelationofstartmatingintervalandthefirstandthesecondpari鑼interval，startmating

4、intervalandthesecondandthethirdparityinterval，thefirstandthesecondparityintervalandthesecondandthethirdparityintervalwere012，065，068，respectively2、nepolymorphism(T／C)intheL0ctls102bpaffectingtheCOWS’fertilitywasfhstlydet

5、ectedintheLeptingeneexon2area(E2FB)，afterSequencingFortheaverageparityinterval，thereweresignificantdifferencesbetweengenotypeAAandgenotypeAT(P鉚01)betweengenotypeATandgenotypeTT(PD05)，However，thereweresignificantdifferenc

6、esbetweengenotypeAB，genotypeBCandgenotypeAA(P001)5、Thepolymorphism(T／C)inthelocus273bpaffectingtheCOWS’fertilityWasdetectedintheESRagene’Sexon1areaFortheaverageparityinterval，thereweresignificantdifferencesbetweengenotyp

7、eCC，genotypeCDandgenotypeDD(P卯01)6、LeptingeneandESRgenescouldbeusedasmoleculargeneticmarkersinfertilityofHolesteinCOWSThroughprimaryresearchonfertilityinfluencedbyleptinandESRbyUSeofPCRSSCPmethod，severalnewSNPsWasfoundfi