水稻生物信息數(shù)據(jù)庫及本地化生物信息分析平臺的構(gòu)建.pdf

1、華中農(nóng)業(yè)大學(xué)碩士學(xué)位論文水稻生物信息數(shù)據(jù)庫及本地化生物信息分析平臺的構(gòu)建姓名：張利達(dá)申請學(xué)位級別：碩士專業(yè)：生物化學(xué)與分子生物學(xué)指導(dǎo)教師：張啟發(fā)2002.5.1AbstractⅥ’euselarge—scaleESTssequencingtostudylevelsofgeneexpressionassociatedwithcultivatedtraitsToperformlarge—scaleanalysisofthelargeamou

2、ntsofthesequencesanddiscovernewgenes，weconstructedanintegratedbioinformaticanalysissystem1ConstructionofanintegratedESTsanalysissystembasedonlocalBasedonLinuxoperatingsystem，thePhred／Phrap／ConsedsoftwareandBLASTsoftwarew

3、ereusedtoconstructaplatformforbatchanalysisofthesequences，includingBasecalling，contaminateremoving，repeatsequencesmasking，sequenceassemblyandsequencesimilarityanalysisMoreovergeneidentificat!on，transcriptioncontrolregion

4、analysis，multiplesequencealignment，phylogeneticanalysis，simplesequencerepeatidentificationandprimersdesigningarealsoincludedOnthebasisofthese，WebinterfaceofBLASTsoftwarewasconstructedforvisualizedsequencesimilarityanalys

5、is，alloftheusersintheintranetcouldvisittheWebBLASTserverusingbrowsertoanalysistheirsequenceeasily2StudyinESTclusteringmethodandapplicationinriceWedevelopedanESTclusteringmethod，ESTClustering，togeneratehigh—qualityuniquee

6、xpressedsequencebasedonlarge—scaleESTsequencingThemethoduseconsensussequencestosequenceanalyzewithmegablastandassembleeachclusterwithphrapinclusteringprocessTheclusteringstrategycanefficientlyidentifygonefamilyandalterna

7、tesplicingformsofexplesscdseqtlcncesItcanalsoreducetheadverseeffectscausedbysequenceerrors’lheFS1Clustm’ingmethodtendstoprovidemoreexpressedgoneformscomparingwiththe【lniGcneclusteringmethodoftheNationalCenterlbrBiotechno

8、logyInformationAnalysisofthe112256ESTsofA／‘a(chǎn)bidopsiswithESTClusteringproduced23，581ESIclustersAmongtheseArabidopsisESTclusters，13597havecorrespondinggenomecodingsequencesandthisnumberisclosetothenumberofgenespredictedwit