SSR分子標記在玉米遺傳育種中的應用.pdf

1、內(nèi)蒙古農(nóng)業(yè)大學碩士學位論文SSR分子標記在玉米遺傳育種中的應用姓名：劉志雄申請學位級別：碩士專業(yè)：作物遺傳育種指導教師：白晨馮勇20090501ApplicationofSSRmolecularmarkeringeneticandbreedingofmaizeAbstractThe34maizeinbredlinesinthispaperwerefromMaizeResearchCenterInnerMongoliaAcademyofA

2、griculturalSciencesandotherplaces90crosscombinationsweredesignedwiththemethodofincompletediallelteamandcorrelationanalysisonthematerialswascarriedoutaccordingtothetotalvalueofcombiningGeneticdistancesofthe34inbredlineswe

3、reresearchedwithSSRmolecularmarkertechnologybasedonthe16fieldcharacteristicsofinbredlinesSomehigherpolymorphicSSRprimersinmaizeinbredlineswereselectedResultsofclassificationmethodswerecomparedTheresultswereasfollows：1Clu

4、steranalysisofthe34maizeinbredlinesWascarrriedout，basedonthe16fieldcharacteristicsofthem，resultingin4groupsTheresultWassimilartothelinerelationshipofthosematerials2Heterosisgroupsofthe34inbredlinesweredividedbySSRmolecul

5、armarkertechnologyThevariationof45alleleswasfoundoutby20pairsofSSRprimersEachpair’ofprimersscreenedout5～1lalleles，withtheaverageof75alleles，foreverypairofprimersThePICrangedfromO758toO888，謝也theaverageof08263BasedontheSSR

6、fingerprint，the34maizeinbredlinesweredividedinto4groups塒tllW|0rd’Smethods6germplasmswereclassifiedintogroup1，8intogroup2，7intogroup3and13intogroup4Theclasificationresultswerebasicallyindenticaltothosebasedongeographicori

7、ginorgermplasmlineage4Thematerialscarlbedividedwithbothmethods，withsimilarresultsSSRmarkersofgeneticdistancereachedasignificantlevelwithspecificcombiningabilityinF1andtherelevanceofyieldperplantSoitispracticalthatthehete

8、rosisgroupsweredivided、析mSSRmolecularmarkertechnology5AsetofopitionalSSRPCRsystemWasbuilt1minutesofpredenaturationat93℃1minutesofdenaturationat93“C1minutesofannealingat56“C65“C(dependingonthematerials)2minutesofextension

9、at72。C30“35cycles(dependingondifferentmaterials)2minutesofextensionlat72℃5minutesoffmalReextensionat72℃6Somenewbreedinginbredlinesweredividedintoheterosisgroups，andsomehighercombinationswerefoundoutThoseresultsprovidedth