水稻抗白葉枯病候選基因—Xa4的克隆研究.pdf

1、四川農(nóng)業(yè)大學(xué)博士學(xué)位論文水稻抗白葉枯病候選基因—Xa4的克隆研究姓名：王世全申請(qǐng)學(xué)位級(jí)別：博士專業(yè)：作物遺傳育種指導(dǎo)教師：李平翟文學(xué)200305016、迸一一步對(duì)IRBB56的BAC克隆106P13進(jìn)行部分序列分析，發(fā)現(xiàn)其上有12個(gè)與Xa4B高度同源的拷貝。7、在106P13上發(fā)現(xiàn)一個(gè)重復(fù)單位為90bp的重復(fù)序列，該序列廣泛存在于水稻基因組中，PCR擴(kuò)增和序列數(shù)據(jù)庫(kù)搜索發(fā)現(xiàn)該序列是水稻特有的重復(fù)序列。關(guān)鍵詞：水稻基因組抗白時(shí)枯病基因核酸

2、結(jié)合位點(diǎn)富亮氨酸重復(fù)細(xì)菌人I：：1二染色體AbstractPlantdiseaseresistanceisoftencontrolledbyMendeliangenesandfollowsagene—forgenerelationshipinmanyplantspeciesandtheirpathogensTodate，manyresistancegene(Rgene)havebeenclonedRgenesfromdifferents

3、peciesappeartOsharesequencesimilaritiesincertaindomainsoftheirgeneproductssuchasleucinerichrepeats(LRR)andnucleotide—bindingsites(NBS)Bacterialblight(BB)，causedbyXanthomonasoryzaepvoryzae(Xoo)，hasbeenoneofthemostseriousd

4、iseasesinrice，affectingproductioninirrigatedandrain—fedlowlandecosystemsthrou曲outAsia，northernAustralia，mainlandAfricathesouthernpartoftheUnitedStatesandLatinAmericaThegeneticbasisofhc；stresistancetObacterialbligilthasbe

5、enstudiedindepth，andmanyresistancegeneshavebeenidentifiedfromcultivatedriceandwildricespeciesTwooftheseresistancegenes，Xa21andXal，havebeencloned，Inthisarticle，wefoundthataresistanceSequence，RS13，wasCO—segregativewithXa4R

6、S13wasusedtOidentifyBACclonesfrombacterialartificialchromosomelibraryFourandtweheclonesfromdifferentBAClibraryIR64andIRBB56，havebeenidentifiedTwocloneshavebeensequencedandanalyzedHerearethemainresultsIRiceresistancetObac

7、terialblightgene，Xa4，wasmappedbetween56M22Fand26D24RinchromosomeI1Thegeneticdistancebetweenthemwas1IcMandthephysicaldistancewas90kbAresistancegenehomologue，RSl3，wasco—segregativewithit2FourcloneswereidentifiedfromBAClibr