三疣梭子蟹養(yǎng)殖塘免培養(yǎng)細(xì)菌多樣性分析【畢業(yè)設(shè)計(jì)】

1、本科畢業(yè)設(shè)計(jì)本科畢業(yè)設(shè)計(jì)（20_20__屆）屆）三疣梭子蟹養(yǎng)殖塘免培養(yǎng)細(xì)菌多樣性分析所在學(xué)院專業(yè)班級(jí)生物工程學(xué)生姓名學(xué)號(hào)指導(dǎo)教師職稱完成日期年月2摘要：摘要：本文采用免培養(yǎng)微生物學(xué)技術(shù)分析三疣梭子蟹養(yǎng)殖塘水體細(xì)菌多樣性。通過構(gòu)建16SrDNA克隆文庫的方法，調(diào)查三疣梭子蟹養(yǎng)殖塘水體7、8月份微生物群落結(jié)構(gòu)。構(gòu)建2個(gè)16SrDNA克隆文庫，總共得到131條有效序列。結(jié)果顯示，比較于培養(yǎng)法，2個(gè)文庫中細(xì)菌更加多樣化，不但藍(lán)細(xì)菌綱和γ變形桿菌

2、綱變更成優(yōu)勢(shì)種群，獲得了Cyanobacteria等培養(yǎng)法沒有獲得的類群，共分為5個(gè)門（變形菌門（Proteobacteria）、藍(lán)藻門（Cyanobacteria）、放線菌門（Actinobacteria）、CFB類群（CytophagaFlavobacteriumBacteroides）以及厚壁菌門（Firmicutes），所以免培養(yǎng)法可以更加完整的反映養(yǎng)殖塘水體菌落組成。關(guān)鍵詞：關(guān)鍵詞：三疣梭子蟹；養(yǎng)殖塘水體；16SrDNA；微生

3、物群AbstractInthispaperusecultivationindependentofbacterialdiversaityinPtunustrituberculatusrearingpond.hemethoddependingonmolecularbiologytechniques16SrDNAclonelibraryconstructionwasalsousedtoinvestigatebacterialcommunity

4、structureinthesamewaterofPtunustrituberculatusrearingpondinJulyAugust.Bytheconstructionoftwo16SrDNAclonelibrary131effectivesequenceshasbeenobtained.Theresultsshowedthatrelativetothemethodofmicrobiologicalculturethedivers

5、ityofbacteriaintwoclonelibrarywasrichernotonlyasthedominantgroupsofbacteriaturnedintocyanobacteriaγProteobacteriabutalsoobtainedsomegroupofbacteriasuchasCyanobacteriawhichdidn’tobtainedwiththemethodofmicrobiologicalcultu

6、re.Allofthebacteriacouldbeattributedtofivephylums(ProteobacteriaCyanobacteriaActinobacteriaCFBgroup(CytophagaFlavobacteriumBacteroides)Firmicutes).ThedominantgroupsofbacteriawerecyanobacteriaγProteobacteria.Soitisobvious

7、lythatbyusingthemethoddependingonmolecularbiologytechniqueswithoutmicrobiologicalculturecouldreflectthecommunitystructureofbacteriainwaterofrearingpondsmecomprehensive.KeywdsPtunustrituberculatusAquaculturepond16SrDNAMic