杏鮑菇蛋白提取方法的研究

1、杏鮑菇蛋白提取方法的研究摘要摘要以杏鮑菇副產(chǎn)物為原料通過干燥、粉碎、堿提、酸沉、回調(diào)、凍干等工藝提取杏鮑菇蛋白質(zhì)。研究了pH值、固液比、提取溫度和提取時(shí)間等單因素對蛋白質(zhì)提取率的影響并經(jīng)正交試驗(yàn)優(yōu)化提取工藝條件。結(jié)果表明：杏鮑菇蛋白質(zhì)適宜提取工藝條件為pH值11.0、固液比1：40（gmL）、提取時(shí)間40min、杏鮑菇蛋白質(zhì)提取率為（42.371.63）%、純度（63.40.87）%。關(guān)鍵詞：杏鮑菇；蛋白質(zhì)；破提AbstractUsin

2、gPleurotuseryngiibyproductastherawmaterialbythetechnologyofdryingcrushingalkaliextractionacidprecipitationcallbackfreezedryingthePleurotuseryngiiproteinwasextracted.TheeffectsofpHvaluesolidliquidratioextractiontemperatur

3、eextractiontimeonproteinextractionratewerestudied.thethogonaldesignwasusedtooptimizetheextractionconditions.TheresultshowedthattheoptimumconditionofextractionprocessfPleurotuseryngiiproteinwasatpH11.0solidliquidratio1:40

4、(gmL)extractiontime40min.UnderthisconditiontheextractionrateofPleurotuseryngiiproteinwas(42.371.63)%theproteincontentwas(63.40.87)%.Keywds:Pleurotuseryngiproteinalkaliextraction目錄前言.......................................

5、...............11.材料與方法..............................................11.1.原料與試劑..........................................11.2.儀器與設(shè)備..........................................11.3.試驗(yàn)方法......................................

6、......22.結(jié)果與分析..............................................32.1.牛血清白蛋白標(biāo)準(zhǔn)曲線................................32.2.單因素試驗(yàn)..........................................42.3.正交試驗(yàn)結(jié)果........................................63.總結(jié).....