玉米花粉多糖對(duì)豬瘟弱毒苗免疫效果的影響研究.pdf

1、廣西大學(xué)碩士學(xué)位論文玉米花粉多糖對(duì)豬瘟弱毒苗免疫效果的影響研究姓名：溫納相申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：傳染病學(xué)與預(yù)防醫(yī)學(xué)指導(dǎo)教師：陸芹章2000.6.1StudiesoneffectofcornpollenpolysaccharidesforimmuneResponsetoAttenuatedHogcholeravirusChineseCstrainAbstract：Onthisstudyweusedalcoholprecipitation

2、toextractive3509ofcornposen，andacquired3889ofcrudeextractsAfterusedcrudeexffactdo(anthraone，molishseliwanoffiodide)reactionandhighpressureliquidchromatographtestidentificationcanvalidationthatit“SpolysaccharidesExperimen

3、talgroupswerevaccinatedwith①50ragpolysaccharidesHCLV；②100mgpolysaccharidesHCLV；③200mgpolysacchmidesHCLV；④physiologicalsolinesolutionHCLV；(eveygrouphavethreenonimmunepigs)everyweekabstractionrespectively；thendeteminecelli

4、mmunityandhumoralimmunitystandardTheresuRsshowed①inktest：antibodylevelofexperimentalgroupallhigherthancontrolgroupThoughttestif00s)significanceofdifference②TlymphocyteactiveErosettetest：Thepersentof們fiveErossettofexperim

5、entalgroupare：2184％2546％2192％allhigherthancontrolgroup1591％Throughttest(PO05)significanceofdefference；③Tlymphocytetransformationtest：thetransformationrateofexperimentalgroupare5532％，56390，5427％allhigherthancontrolgroup47

6、00％，andnotaddPHAgroup134％Throughttest口005)signigieanceofdefference；④Tlymphocytesubgroupassay：theexperimentalgroupsofavergevalueTlympocyteCD3、CIM、CD8allhigherthamthatofcontrolgroups，buttheNKcellofexperimentalgroupsandcont

7、rolgroupwerenotsignificanceofdifferenceThebestdossagewas100mg／pig，thenuSeittodofieldtrialtheantibodylevelwasmeasuredwithIHAtesttheresultswerecoincidentwithlaboratorytestThestudiesofresultdemonstratedthatcornpollenpolysac