秦艽發(fā)根系體細胞胚的誘導和植株再生.pdf

1、蘭州大學碩士學位論文秦艽發(fā)根系體細胞胚的誘導和植株再生姓名：李燕申請學位級別：碩士專業(yè)：生物學、細胞生物學指導教師：王新宇20080501蘭州大學碩士學位論文AbstractSomaticembryogenesis(SEM)isoneofplantregenerationinvitro，andisveryusefulfornotonlystudyingthemechanismofplantembryogenesisandcelldiff

2、erentiation，butalsoscreeningnewvaluablecropvarietyviacellandgeneengineeringmethodHairyrootsisakindofrootlikeorgan，whichproducedfromthewoundplantportioninfectedbyAgrobacteriumrhizogenesInsomepropercultures，hairyrootscould

3、beinducedtoproducecallus，evenwholeplantletsBecauseoftheplasmidRiinserted，somenewplantswithexcellectcharaterscouldbegoRenGentianamacrophyllaPallisawidelyusedastranditionalherbalmedicinebecauseofcontaininggintiopicrinTheco

4、mpoundsynthesizedandaccumulatedinitsrootwasreportedtoshowmedicinalfunctionsincludingabirritation，anti—bacterium，antiinflammatoryandantipyreticactivities，andhavebeenusedtocurevariousdiseasesInordertealizethepurposeofreaso

5、nableandlongtimeultilizationofthislimitedresource，usinghairyrootsofGentianamacrophyllaPall，weestablishedaefficientsystemofplantregenerationthroughsomaticembryogenesisAtthesametime，theeffectsofplanthormonesonsomaticembryo

6、sformationwasstudied，andtheconditionsforhairyrootinductionwereoptimizedMainresultsfollowas：30daysafterculturedonsolidMSmediumwith01～100mg／L24一D，allthehairyrootsegmentsofGentianamacrophyllaPall，producedbyinfectingwoundedc

7、otyledonwithAgrobateriumrhizogenes，convertedintoyellowishcompactembryogeniccalliAftertransferredintohormonefreeMSmedium30days，thesecallidevelopedintolooseyellowishcalliconsistingofmanysmallcompactparticles(globalsomatice

8、mbryos)More30daysaftercutintosmallerpiecesandtransferredontofreshMSagarmediumwithouthormone，manysingleandfasciculateshootsformedonthesurfacesofthesecalliUndertheanatomicalmicroscopythesecultureswerefoundcontainingmanyglo