基于氣相色譜技術(shù)的尿糖探針和血清游離脂肪酸檢測研究.pdf

1、A Dissertation Submitted in Partial Fulfillment of the Requirementsfor the Degree of MasterThe Detection of Sugar Probes inUrine and Free Fatty Acids in SerumBased on Gas ChromatographyCandidate : Zuo Qi MinMajor :Tradit

2、ional Chinese MedicineSupervisor : Assoc.Prof. Jiang MingHuazhong University of Science and TechnologyWuhan, Hubei 430074, P. R. ChinaMay, 2017目 錄摘 要.......................................................................

3、.......................................................1Abstract.........................................................................................................................2第一章 第一章 氣相色譜法同時測定尿液中甘露醇等 氣相色譜法同時測定

4、尿液中甘露醇等 5 種糖的研究 種糖的研究..................................41.前言.........................................................................................................................42.實驗部分................................

5、.................................................................................42.1 實驗試劑..........................................................................................................42.2 實驗儀器...............

6、...........................................................................................52.3 儲備溶液與對照品溶液制備..........................................................................52.4 尿樣前處理............................

7、..........................................................................52.5 衍生化處理......................................................................................................52.6 氣相色譜條件.......................

8、..........................................................................62.7 方法學驗證......................................................................................................62.7.1 線性和檢測限.....................

9、..........................................................................62.7.2 精密度...........................................................................................................62.7.3 加標回收率.................

10、..................................................................................62.7.4 樣品穩(wěn)定性...................................................................................................73.實驗結(jié)果....................

11、.................................................................................................73.1 衍生化方法的優(yōu)化..............................................................................................73.2 方法學驗證......

12、....................................................................................................83.2.1 線性和檢測限...............................................................................................83.2.2 精密度..

13、.........................................................................................................93.2.3 加標回收率.................................................................................................103.2.

14、4 樣品穩(wěn)定性.................................................................................................104.討論..............................................................................................................

15、.............10第二章 第二章 串聯(lián)毛細管色譜柱法用于血清游離脂肪酸的檢測 串聯(lián)毛細管色譜柱法用于血清游離脂肪酸的檢測....................................121.前言................................................................................................................