流加操作輔助變性溶菌酶復(fù)性.pdf

1、天津大學(xué)碩士學(xué)位論文流加操作輔助變性溶菌酶復(fù)性姓名：李琳申請學(xué)位級別：碩士專業(yè)：生物化工指導(dǎo)教師：董曉燕20030101AbstractThisworkstudiedthecharacteristicoftherefoldingkineticsofdenaturedIysozymewithfedbatchoperationsystemicallyTherefoldingprocessmodelwasconstructedandfurth

2、ermorethemodelwasrevisedatahighlysozymeconcentration，Inaddition，twepresentativeadditives，acetamideandglycerin，whichmightbeappliedintheindustrialproductionofproteinrefolding，werealsostudiedforthepruposeofassistingdenature

3、dlysozymerefoldingTheresultlndicatedthattheproteinconcentrationcouldbecontrolledatlOWleveIduetothecharacteristicofthefedbatchoperation，whichwasdilutingandrefoldingatthesametimeItcoulddecreasethehydrophobicroleamongtheden

4、aturedproteinmoleculesIntheconditionofhighlysozymeconcentration，thequantityofoxidantmustbeincreasedtoenablethedenaturedandreducedlysozymetorefoldintimeWhenthehighconcentrationofGdmClinhibitedtherefoldingreaction，usingthe

5、fed—batchoperationanddialysistogethercanrealizetheproteinrefoldinginhighconcentrationThefed—batchrefoldingprocessmodel，whichwasconstructedbasedOilthirdorderapparentkineticreactionequationcouldsimulateandforecasttheexperi

6、mentalrefoldingkineticprocessonlyatthelowlysozymeconcentrationWhenthelysozymeconcentrationwashigh，theforthorderapparentkineticreactionequationshouldbeusedtOobtainthesatisfactoryresultIntheresearchofadditivesassistingther

7、efoldingofdenaturedlysozymewithfed—batchoperation，theresultindicatedthatbothoftheacetamideandglycerincouldassistthedenaturedlysozymerefoldingtogetahigherrefoldingefficiencytogetherwithfed—batchoperationAcetamidecouldinhi

8、bittheaggregationformationbyinhibitingthehydrophobicreactionoccurredamongthepeptidechainsWhentheGdmClconcentrationwasdecreased，theproperincreasingofacetamidecouldassistthelysozymerefoldingefficientlyHoweverthemechanismof

9、glycerin，whichassistedtherefoldingofdenaturedlysozyme，wasnotsametoaeetamide，itenhancedthethermokineticstabilityofnatureproteinandfacilitatedthereactionmovingtotheproductionofnatureprotein，butitcouldn’tinhibitthehydrophob