白腐菌產(chǎn)漆酶及其在染料降解中的應(yīng)用.pdf

1、華中科技大學(xué)碩士學(xué)位論文白腐菌產(chǎn)漆酶及其在染料降解中的應(yīng)用姓名：梅運(yùn)軍申請學(xué)位級別：碩士專業(yè)：生物化工指導(dǎo)教師：魯明波2003.5.10華中科技大學(xué)碩士學(xué)位論文==Il：=自=l____；jtI_目I_目________I_l__AbstractPanusconchatus，akindofwhiterotfungi，hasahighlaccaseproductivityIthasbeenwidelyresearchedintheslur

2、ringtechniqueandtreatmentofwastedwaterinthepapermakingindustryInpresentstudythecultureconditionssuitableforlaccaseinPanusconchatusweteestablishedfirst；andthentheeffectofmetaliononlaccaseactivityWasexaminedInaddition，magn

3、ifiedcultureoflaccaseWasconductedbymeansofmicrobialfermentationThedegradationactivityoflaccasewasstudiedin2differentwaysatalablevel，oneofwhichWasprocessedinswingingbottlebyselfimmobilizedPanusconchams，whiletheotheradopte

4、dbio—membranetechniqueResuitsoftheabove—mentionedstudiesindicatethat：1TheoptimumculturemediumforlaccaseproductionanddyedegradationbypanusconchatuswerestudiedTheresultsshowedthattheoptimumcultureconditionswereglucose10VL，

5、ammoniumtartrate059／L，pH30，rotationspeed120ffmin，Tween8004％，CU2041amol／L，underwhichtheactivityofLaccasereached16234U／mL2Basedontheexperimentabove，themagnificationofmicrobialfermentationforlaccaseproductionWasrealizedLacc

6、asebiosynthesisbypanusconchatusWaSenlargedina5LBIOSTATB，witharotatingspeedof120r／rain，agasvolumeof48m3／h，Tween80O5％Undersuchconditionsthedissolvedoxygenconcentrationandtheoxygentransmiaingcoefficientwerehigh，whichWaScond

7、ucivetoattainahi911productionandactivityoflaccase，witllthelatterachieving13296U／mL3TheeffectofmetaliononlaccaseactivitywasstudiedTheadditionofO4umoI／LCu2madelaccaseactivitythehighestTheadditionofH92hadacertaineffectonlac