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1、東北林業(yè)大學(xué)碩士學(xué)位論文大青楊多倍體的誘導(dǎo)姓名：祁傳磊申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：林木遺傳育種指導(dǎo)教師：李開隆201106AbstractAbstractFemalecatkinsweretreatedwith02％～06％colchicinesolutionduring12～48hrspollinationincrosscombinationsbetweendialingpoplarweihepoplar，Somehybridswereo

2、btainedbytreatingthepollinatedflowerswithcolchicinesolutionandwithoutsolutioncolchicinesThenthehybridsbytreatingthepollinatedflowerswithoutcolchicineweretreatedwith01％～04％colchicinesolutionAfterfinishingtheaboveprocess，S

3、OWallseedatthesametimeWaterandnutrientsweremanagedduringthewholegrowth12triploid(the#lmtedflowersbeingtreatedwithcolchicinesolution)and24tetrapioid(theseedbeingtreatedwithcolchicinesolution)plantswenobtainedthroughchromo

4、somenumberingoftheplanttipcellbeingconductedandDNAcontentinleafcellsbeinginvestigatedInordertoinvestigatethereasonsfordifferencesamongvariousploidyPopulusussuriensisingrowthtraits，thecomparativestudyonleafstructureandpho

5、tosynthesischaracteristicsoftwoyearolddiploidtriploidandtetrapioidPussuriensiswasexaminedTheresultsshowedthat：(1)36“60hrsaflerpollinationwastheoptimalperiodand05％colchicinesolutionⅥ協(xié)stheoptimalconcentrationtoinducetriplo

6、idsinthesecrosscombinations，andthehighestratioofobtainingtriploidswas75％TheresultsshowedthattreatedseedofPopulusmsuriensiswith02％colchicineleadtoahighmutationrate(upto89％)1eavesofthetriploidandtetraploidseedlingswerelarg

7、erandthickerthanthoseofthediploidones，andthestomatalsizeanddensityontheleaflargerandthickerthanthatofthediploidrespectivelyThetetraploidplantsexibitedleaflengthwidthandthichnessareincreasedbiggestramongdifferentptoidyeus

8、suriensis(2)DifferentploidyPoputusussuriensisaredifferentsignificantlyincontentsofSolubleproteinandchlorophyll，tetraplontSolubleproteincontentishiger23％thandipolid，teWaplonchlorophyllcontentishigher14％thandipolidThediffe

9、renceofannualdifferentploidypopulusussuriensisisnotsignificantingrowth，111edifferenceofbiennialdifferentploidypopulusussuriensisissifnificantinstemheight，butnotsignificentinbasaldiameter(3)T11ecomparativestudyonleafstruc

10、tureandphotosynthesisoftwoyearolddiploid，triploidandtetrapioidPopulusussuriensisWasexamined，inordertostudythereasonsfordifferencesingrowthtraitsTheresultsshowedthatnetphotosyntheticrate，lightsaturationpointandcompensatio

11、npointamongPussuriensisofdifferentploidyweresignificantlydifferentThevalueofthemaximumphotosyntheticratefromthetriploidplantwas21％lligherthanthediploidTriploidandtetrapioidPussuriensisweresignificantlyincreasedinthicknes

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