應用于生豬飼養(yǎng)的益生微生物的篩選及其生物學性能評價.pdf

1、學位論文獨創(chuàng)聲明學位論文獨創(chuàng)性聲明本人聲明所呈交的學位論文是本人在導師指導下進行的研究工作及取得的研究成果。據我所知，除了文中特別加以標注和致謝的地方外，論文中不包含其他人已經發(fā)表或撰寫過的研究成果，也不包含為獲得南昌大學或其他教育機構的學位或證書而使用過的材料。與我一同工作的同志對本研究所做的任何貢獻均已在論文中作了明確的說明并表示謝意。學位論文作者簽名(手寫蘭嘉拇蕨簽字日期：夕口11年I1月力轤學位論文版權使用授權書本學位論文作者完

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3、actAbstractChinaisthelargestcountryrmsingpigsintheworldAseffectivefeedingadditives，antibioticshavebeenwidelyusedinpigraisingstomeettherequirementsofintensiveandlargescalelivestockbreedingHoweversomeadverseeffects，egdrugr

4、esidues，teratogenesis，carcinogenicmutagenicareemergingcomparedwimtheantibioticabusing，whichmakestheecologicalpigraisingmethodtobetheresearchfocusEcologicalpigraisingmethodreferstoreplacingtheantibiotics誦tllprobioticmicro

5、organismswhenraisepigs，includingtheprobioticspreparationforfermentationbedInrecentdecades，thismethodhasbeenappreciatedbymostresearchersforitsbeneficialeffects，suchasgrowthpromotingwithoutsideeffects，noresidualcontaminati

6、onandnodrugresistanceInordertoinvestigatetheeffectsofprobioticmicrobesonfermentationbedseveralselectedmediaWereusedfortheisolationofmicroorganismscontaininginmicrobialecologicalagents，and6isolateswereobtainedaccordingthe

7、irclonemorphologycellmorphologythenthePCRDGGEtechnologywassubsequentlyusedtoclassifythemandonlytwodifferentstrains，named雒LactobacillusplantrumandCandidatropicalisWereidentifiedInnextstep，theeffectsofthepH，temperature，bil

8、esalts，copperaerobic／anaerobiconthevialblecellcountingofisolatesWereinvestigatedOurresultsshowedthattheLactobacillusplantrumcan’tgrowwhenthepHisblow25orthecopperionconcentrationishigherthan150ppm，whilethenumberofCandidat

9、ropicalisreceiveslittleimpactbysuchfactorsInaddition，theamountsofCandidatropicalisreduced6magnitudesat42“12comparedwiththatat37℃Also，wecheckedtheimpactsofdifferentantibiotics(amoxicillinoxytetracyclinehydrochloride，metha

10、cholinequinoline，olaquindox，clarithromycin，ciprofloxacin，oxytetracycline)onthegrowthofprobioticsandpathogenscommonlyexistedinfermentationbed，andtheconcentrationsofantibioticsWeredeterminedaccordingtotheirfeedingqualityan