水稻轉(zhuǎn)化酶基因家族的進化及其在干旱脅迫下的表達調(diào)控.pdf

1、中國農(nóng)業(yè)科學(xué)院博士學(xué)位論文水稻轉(zhuǎn)化酶基因家族的進化及其在干旱脅迫下的表達調(diào)控姓名：紀(jì)雪梅申請學(xué)位級別：博士專業(yè)：作物遺傳育種指導(dǎo)教師：程式華JohnBent20050101AbstractInvertases(囂c32126)a糟en搿翔es硅latc蹴lyze也ein專versibleh蜘袖lysisofSucfoseto露啪o∞髓df抽coseplaIItscol岫two瑚∞la涮famniesof坩陀r紐ses：acidfom塔f

2、loca主edinmecell—wallandVacuole)酬neu蚓觸kaj婦for麟C岫u蜘tobel礎(chǔ)din齜c腳s01)Here軸如eIi如聰母濂genes船eod殛gtlecellwaIl掣oup(desi鏟ated蕊cw，一甥thVacuolar掣oup(蕊纜w忉粕d腳oancientne曲融櫥kaI沁掣oups：氆(蕊^心強一句andB(蕊腳一∞AnalysisofmegeneSequencesallowsconclus

3、ionstok㈣c∞∞州bg嘲esubcelMarloca畦強of由eencodedproteinsand氆eeVolutionoftheacidandne蛾刪黝ine遺ve批醇egenefhmiliesasreg礬sin的n吒xons蚋Jc撖心，genedu掰ic撕鴨g霉neloss艄d窯eneexpressionComp8redw確曲erel雛ivelyconserv酣s弧lcmreofne曲蛆黼kalineinvertasegen

4、efhmily’出eacidinvertaSe粕!nilyunde州entmanyeventsofin‰nl∞sTbe8c掛西Venase譽嫩esaboshowedgrea把rspa士i越and鈀mpo豫ldiVers毋ofexp糟Ssion搬an舭neul確l，a墩a|ine窖enesIfocusedonexamillngpossible|e船。髂觸’t如躲lltiplic對ofthcacid誠vercasegenefamilySin

5、ce錕l科mesoffhlcta稚S)mmesisareblowntohaveevolved矗nmvacuolari“vertascs，lbeg鼢wi墩偽蹦fand蕊珊睨a積a堪∞dwhe墩crb硪genes翱codeda嘣ve如碰aSesgueosyl一酗ctosidases)orwle壤eronegeneencodeda熱J髓osyl姍sf鼬secapableoffuct柚syn氆eslsA瑚∞ughr主cedoes赫otaccum

6、ulate蠹Ⅵc瓴璐，融cloSelyrelatedcere8重ssuchas橢eatandb翻cy翻ctallsplayallimp0執(zhí)t翔|einto王er毪nceofdroIl曲ta玎dcoldsh岱sIcharac釹zed偽唧，鑫nd0嘧瑚憶intcⅡnsof也een彬maticprope撕esandsequencereia畦0nshipsoftleenc硼edpfotens，andtheirrcgulation_mfespof

7、lsetodfou班s№ssRecombinaIltOsV玎qlandosV夏N2p∞把jnsexp揩ssedin量leyeaSt崩拍缸p甜細(xì)凰showedhighinVenaseac畦vityagainst2mMsucfosc，andon重yiow1sucrose：sucrosef軸ctosyltransfer矗seacⅡv耐(1kestosesynthesis)砒60lOOOmMsucroseFurl量lemo精fecombinan

8、tOsV烈lshowodsi刪蠡cantlevelsof?！辌tosyl蠡Ⅵctosi如a商Vi移ag婦1stl—kestose，l，l—nystoseandi黼lin(舳ctanexohydrolasea娃ivi劬Byc∞tra磚recombina嵇tosV壬N2lacked矗_llct芒mex曲ydrolaSeac廿vi移andcould氆e撐fore托presentamajorevolutiona掣steptowardsachie