分散液液微萃取在擬除蟲菊酯類藥物殘留檢測(cè)中的應(yīng)用研究.pdf

1、河北大學(xué)碩士學(xué)位論文分散液液微萃取在擬除蟲菊酯類藥物殘留檢測(cè)中的應(yīng)用研究姓名：杜精精申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：藥物分析學(xué)指導(dǎo)教師：閆宏遠(yuǎn)2011-06Abstract IIAbstract Sample preparation is an important step in the analysis of actual samples, especially for the determination of trace levels of

2、 analytes in complex matrices. Traditional pretreatment methods are gradually changing to be miniature and economic so as to lower cost and pollution. Dispersive liquid-liquid microextraction (DLLME) is precisely a new s

3、imple, economical and environment-friendly preparation technique. This work investigated the extraction efficiency of DLLME and improved its applications in complex samples, with the pyrethroids as the representative ana

4、lytes. Firstly, this work summarized the research of the extraction-separation techniques in sample pretreatment, and outlined the development and applications of DLLME. Then, DLLME combined with gas chromatography (GC)

5、was developed for the simultaneous determination of nine pyrethroids in river water by means of ultrasound-assisted emulsification, which markedly improved the extraction efficiency and shortened the time. Based on that,

6、 the real samples were further extended to more complex biological samples (pear). The factors which had significant impact on the extraction efficiency were investigated and optimized. Methodological study showed that t

7、he method was simple, rapid and environmentally friendly with less organic solvent. Finally, the combination of solid-phase extraction (SPE) and DLLME, coupled with gas chromatography-electron capture detection (GC-ECD)

8、was developed to overcome the drawback of DLLME and establish a new pretreatment method that can selectively enrich, purify and detect trace drug residues in more complex samples. The SPE sorbents, washing solvent and el

9、uent type, loading volume, eluent amount, extractant and dispersant dosages were optimized. Methodology validation and practical detection indicated that the enrichment factor, purification effect and detection limits of