偶氮類色素半抗原與人工抗原的合成及應(yīng)用研究.pdf

1、南開大學(xué)碩士學(xué)位論文偶氮類色素半抗原與人工抗原的合成及應(yīng)用研究姓名：劉亭亭申請學(xué)位級別：碩士專業(yè)：有機化學(xué)指導(dǎo)教師：王文虎201205AbstractAbstractEdibledyesarewidelyusedfoodadditivesinfoodindustryforimprovingfoodcolorandlusterThefrequentlyusedEdibledyesaresyntheticedibledyes，andmost

2、oftheseareazodyes，whichbystudiedinducehumanlesions，arepotentialcarcinogenicInrecentyears，thefactofazo—ayesusedlawlesslyinfoodhasaseriousimpactonfoodsecurityAtpresent，thestandardmethodtodetectazodyesinfoodisinstrumentsmet

3、hod，becauseithasthesuperiorityofhighaccuracyandlowdetectionlimitAtthesametime，thismethodhasalotofdisadvantages，eg，expensivecost，time—consuming，etcTheenzyme—linkedimmunosorbentassay(ELISA)methodisasensitive，rapid，simpleop

4、eration，cheepmethod，SOithaswideapplicationprospectUptonowonlyafewofazodyeswhichcontainactivegroups(eg，COOH，NH2)canbedetectedbyELISAmethodItisindispensibletostudyandestablishnewELISAmethodsofazo—dyesThekeytoestablishnewEL

5、ISAmethodisthesynthesisofexcellenthaptensandrelatedartificialantigensInthisdissertationweestablishednewroutestosynthesizehaptensofazodyesWesuccessfullysynthesizedfivehaptensofSunsetYellow(18、21(ac)、24)andfivehaptensofAll

6、ureRed(29、32(a—c)、35)ThroughacylationofhydroxylgroupofazodyeswealsosynthesizedthehaptensofsudanredI，acidorangeII，brilliantyellowsunsetyellowandallurared(60a60e)，respectivelyTheresultsseRleagoodfoundationfortherelatedELIS

7、AmethodThestructuresofallthenewfiftyonecompoundswereconfirmedby1HNMRandHRMSInaddition，thecrystalstructureofcompound47ewasconfirmedbyXraysinglecrystaldiffractiondeterminationKeywords：foodsecurityazo—dye，ELISAmethod，hapten