具有抑菌活性成分中草藥的篩選及防腐保鮮應用機理研究.pdf

1、華南理工大學碩士學位論文具有抑菌活性成分中草藥的篩選及防腐保鮮應用機理研究姓名：賴毅東申請學位級別：碩士專業(yè)：食品科學指導教師：寧正祥20030501華南理工大學工學碩士學位論文AbstractThirtyspeciesofChineseherbalmedicineswereextractedwithethanolandtheroughextractsweretestedfortheirantimicrobialandfungitoxi

2、tyactivityonsevenspeciesmicroorganismsThemethodofinhibitoryareawasusedtotesttheinhibitoryeffectsofthirtyspeciesofplantextractsonmicroganismsandsporegerminationofAspergillusnigerandPeronophythoralitchichenResultsshowedtha

3、tfivespecies，RhizomaAcoriRhizoneEugeniacaryophyllataThumb，爿fpiniaofficinarumHanceCinnamomumJaponicum，Rheumpalmatum上havethebestantimicrobialandfungitoxityeffectThefivespecies’ethanolextractshaveshowedstronginhibitoryeffec

4、tsinmycelialgrowthinhibitiontestandinhibitoryareatestfortheirinhibitoryeffectsonsporegerminationofAspergillusnigerandPeronophythoralitchichenalsoshowstornginhibitoryeffectTheinhibitionrateofthefivespecieswereabove85％Thre

5、especies’ethanolextracts，RhizomaAcoriRhizone，EugeniacaryophyllataThumb，CinnamomumdaponicumshowedcompletelyinhibitionrateonmyeelialgrowthandthezoosporesreleaseofPeronoph)’thoralitchichenshowedequivalentstronginhibitoryeff

6、ectswiththechemicalcontrol02％sodiumbenzoateTenmatchingliquorsweregeneratedbytheabovefiveextractionsInhibitoryeffectsofextractionsandmatchingliquorsweremadebymeansofthemiddleinhibitoryconeentration(MIC)，thetestsshowedthee

7、thanolextractsofclove(TW15)andthematchingliquor，PW6havethestrongestinhibitoryeffectonmycelialgrowthandthezoosporesreleaseofPeronophythoralitchichenMICofPW15ishalfofTW15ThephysicalandchemicalcharactsofTW15andPW6weretested

8、afterbeingtreatedatdifferenttemperature，illuminationandpHvaluesAndthetimelengthsforpreservationandtheactivitymaintenancetimelengthsoftwoliquorsTheresultsshowedthatmatchingliquorPW6havebettereffectsthanTWl5inthesetestingi

9、temsTheinhibitoryactivityofTWI5decreasedsharplywhentemperaturehigherthan60℃，TW二l5andPW6remainedmostactiveatpH，bothincreasinganddecreasingofpHcausedthedroppingdownofinhibitoryactivityAftertreatedwithilluminationfor72h，bot

10、hofextractshadnosignificantchangeintheirinhibitoryactivitiesThepreservativeexperimentresultsofTW15andPW6onpeargrapeandlitchishowedthattwopreservativeliquorshavereallysignificantfresh—keepingeffectsComparedtothecontroltes