山仙顆粒對(duì)H-,22-腹水型瘤株移植瘤小鼠IGF-Ⅱ和IGF-ⅡR影響的實(shí)驗(yàn)研究.pdf

1、陜西中醫(yī)學(xué)院碩士學(xué)位論文山仙顆粒對(duì)H腹水型瘤株移植瘤小鼠IGFⅡ和IGFⅡR影響的實(shí)驗(yàn)研究姓名：吳夢(mèng)茹申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：中西醫(yī)結(jié)合臨床指導(dǎo)教師：王希勝20090401TitIe：TheexperimentedresearchwhichshanxianparticIeseffectedtheexpressionofIGF—IIandIGFIIRinKunmingmiceTransfectedbyH22HepatoceIIUIarCa

2、rcinomaAbstractObjective：ThroughtheresearchoftheindexofIGF—IIandIGF—IIRof1ivertransplantationinmiceofshanxianparticles，F(xiàn)urtherclarifytheanti—’cancermechanismofshanxianparticleandprovidebasisforclinicalapplicationMethods：

3、50Kmmicecompletelyrandomweredividedinto5groups，controlgroup(A)，Modelcontrolgroup(B)，Shanxianparticlesofhigh—dosetreatmentgroup(C)，Shanxianparticlesoflow—dosetreatmentgroup(D)，cyclophosphamidethecontrolgroup(E)，Eachgroupo

4、f10Controlsgroupdidnotbevaccinatedtumor，therestofH22laboratorymicewereinoculatedlivertumorcellsuspension1iquidAfterthesuccessofthemodel，dailywithsalinegavagetogroupAandBaccordingto02mlfor14days：IngroupCwereadministratedd

5、osagesofshanxianparticlesbygastricgarageaccordingtol169／kg。dfor14days；IngroupDwerefeedaccordingto299／kgdfor14days：IngroupEwi11beadministrateddosagesofCyclophosphamidebygastricgavageaccordingto30mg／kgd，for14daysEverydayob

6、servedtheappetite，activities，mentalstate，defecateandurinate，fur，deathandweighingofmice，DetailedlyrecordedtheseconditionsAllmiceweresacrificed15dayslater，Gathermice’Stumortissue，thethymusandspleenandweightedthem，calculate

7、dtheinhibitingtumorgrowthrate，theindexofthymusandspleen，UsingimmunohistochemistrydetectedtheexpressionlevelofIGF—IIandIGF—IIRoftumorcell，andanalyzedtheirpositiveexpressionrateandcontentResults：Miceiseatinggood，agile，glos