中藥草豆蔻、黃芩活性成分的光譜性質(zhì)及熒光分析法研究.pdf

1、河北師范大學(xué)碩士學(xué)位論文中藥草豆蔻、黃芩活性成分的光譜性質(zhì)及熒光分析法研究姓名：孟曉彩申請學(xué)位級別：碩士專業(yè)：無機(jī)化學(xué)指導(dǎo)教師：魏永巨20080508AbstractFluorescencespectraandabsorptionspectraofChineseherbalmedicineA勿伽砌katsumadafHayata(agnCaodoukou)，ScutellariabaicalensisGcorgi(SBG，Huangqi

2、n)andⅡleiractivecomponentsalpmetin，cardamoIli瑪baicalinbaicaleinandscutellareinhadbeenstudiedBasedontheirspectralproperties，fluorimetricmethodsfortheanalysisofalpinetinandcardamoninin腳aswell蠲baicalininSBGhadbeendevelopedB

3、ycomparingthefluorescencespectraoftenflavonoids，relationshipbetweenfluorescenceandmolecularstructureWaSdiscmsedThemaincontentsofthethesiswere鶴follows：1SpectralpropertiesofAKHanditsactivecomponent，andfluorimetricanalysiso

4、falpinetinandcardamoninin舢sampleFluorescenceandabsorptionspectraofalpinetinwerestudiedInfluencesofpHandsolventonfluorescencewereinvestigatedUsingLTrp嬲areference，fuorescencequantumyieldofalpinetinWasdeterminedtobe0033atex

5、citationwavelength290nlrlunderpH53111eionizationconstantWasdeterminedtobepKa=696byanabsorptionmethodCardamoninhadbasicallynofluorescenceAfterheating3hoursatl000CunderpH53，cardamonincouldbecompletelytransformedintoalpinet

6、inTheionizationconstantsweremeasuredtobepKal=706andpKa2=984，byanabsorptionmethodAfluofimetricmethodforthedeterminationofalpinetinandcardamoninin4K日wasproposednecontentofalpinetinandcardamonininAK日sampleweredeterminedtobe

7、059％and044％respectively2SpectralpropertiesofSBGanditsactivecomponent，andfluorimetricanalysisofbaicalininsBGsampleBaicalinhadveryweakfluorescenceAtieradditionofborax，fluorescencepeakswerefoundat325／420nlnand270／420砌Usingq

8、uininebisulfateasareferenceandtheconcentrationofboraxWas001moll～fluorescencequantumyieldofbaicalinWasmeasuredtobe0018atexcitationwavelength330nlnⅥmentheconcentrationofboraxWaS001moll～a1inearrelationshipbetweenfluorescenc

9、eintensityandbaicalinconcentrationWaSfoundThecontentofbaicalininSBGsampleWaSdeterminedtobeintherangeof802％～895％。Thefluorescenceofbaicaleinandscutellareinwereveryweekbeforeandafteradditionofborax，SOtheyshouldnotinterferew

10、iththeanalysisofbaicalinin船G3RelationshipbetweenfluorescenceandmolecularstructureoffavonoidWaSdiseased，bycomparingthreedimensionalfluorescencespectraoftenflavonoidsKeywords：Alpinetin；Cardamonin；Baicalin；Baicalein；Scutell