孕期不同階段鉛暴露對大鼠胎盤血栓調(diào)節(jié)蛋白、核因子-κB表達影響的實驗研究.pdf

1、青島大學碩士學位論文孕期不同階段鉛暴露對大鼠胎盤血栓調(diào)節(jié)蛋白、核因子κB表達影響的實驗研究姓名：鄒翠麗申請學位級別：碩士專業(yè)：遺傳學指導教師：王云英20100603ABSTRACTObjectiveToinvestigatethetoxicityofleadexposureontheplacentaatdifferentdosagesandtherelationshipwithplacentalexpressionofNF—rJ3and

2、thrombomodulinMethods108wistarratswererandomlydividedintofourgroups(A，B，C，D)forconsumptionofwaterwithorwithoutO025％leadacetateduringvariousgestationalperiods；Bloodleadcontentwasdeterminedbyatomicabsorptionspectrophotomet

3、ryPlacentalNF1cBandthrombomodulinexpressionwasevaluatedbyimmunohistochemistryTMmRNAWaSevaluatedbyfluorescentquantitativerealtimePCRResultsMaternalbloodleadlevelsintheratswassignificantlyhighercomparedtocontrols(POO1)Mate

4、rnalbloodleadlevelsintheratsnegativelycorrelatedwithplacentalweight(r=0652PO01)Ontheotherhand，placentalexpressionofNFrd3inleadexposedratsWassignificantlyhigherthanthatincontrols(P0O1)TheplacentalexpressionofTMmRNAinleade

5、xposedratswascorrespondingwiththeexpressionofTMproteinThetendencyWasincreasedcontinuousingroupB，groupCandgroupDTheTMexpressioningroupBandgroupCwaslowerthaningroupA，whereastheexpressioningroupDwashigherthaningroupA(P0O1)C

6、onclusionLeadexposureatvariousgestationalperibdsproducevariedeffects，withNF一1(BactivationfollowingleadexposureInjurytocytoplasmicorganellesmayinterferewiththenutritionandoxygenexchangebetweenmotherandfetus，whichmaybecont

7、ributetoabnormalpregnancyoutcomesOntheotherhand，LeadexposureonratplacentalmayaffecttheexpressionofthrombomodulinThiseffectcouldbeassociatedwithimpairmentintheplacentaandmayrepresentonemechanismbywhichprenatalleadexposure