微納流控芯片在藥物和生物分析中的應用研究.pdf

1、浙江大學理學院碩士學位論文微納流控芯片在藥物和生物分析中的應用研究姓名：廬華申請學位級別：碩士專業(yè)：分析化學指導教師：何巧紅20100310浙江大學碩七畢業(yè)論文Abstract摘要NowadaysthebiologicalandmedicaldiagnosisrequiresmoreadvancedanalyticalsystemsWhenitcomestothisproblem，MicrofluidicsandNanofluidics

2、appeartobepromisinganalyticaltoolsInmyworks，theyaleaboutpreconcentratingandseparatingbiologicalandmedicalmoleculesusingmicroandnanodevicesDaunombicin(DAU)andDoxorubicin(DOX)wereseparatedbycrossshapemicrochannelsFITC—BSAa

3、ndFITCwerepreconcentratedbyhybridmiro—andnanochannelsItwouldbefantastictocombinethepreconcentrationpartwithseparationparttoanalyzethebiologicalandmedicalmoleculesoflOWconcentrationInthefirstchaptervariousfabricationmetho

4、dsofhybridmicro—andnanochannelswereintroducedAndthereportsonthepreconcentrationtechniquesofmicrofluidicsandnanofluidicsaswellastheelectrophoresistechniquesofnanofluidicswerereviewedInthesecondchanpterCELIFwasemployedfort

5、heseparationofanti—tumormedicineTheDAUandDOXarenaturallyfluorescentwhichwerefavorabletotheexperimentsAdding40％acetonitriletotheboraterunningbuffersignificantlyimprovedtheseparationefficiencyandthefluorescencedetectionsen

6、sitivityDAUandDOXwereseparatedsuccessfullyinthehumanserumsolutionInthethirdchapterthedoublelithographyanddoublechemicalwetetchingmethodWasusedtofabricatethehybridmicro—andnano—devicesThesedeviceswereappliedtothepreconcen

7、trationofFITC—BSAandFITC，sincetheoverlapofthedoubleelectricallayersFITC—BSAwaspreconcentratedusingthedepletionregion，whiletheFITCWaspreconcentratedintwowaysincludingthewayusuallytopreconcentratesmallmoleculesandthewaysim