自然流產(chǎn)小鼠胎盤細胞凋亡及其相關調(diào)控基因Fas-FasL，Bcl-2-Bax的表達.pdf

1、山西醫(yī)科大學碩士學位論文自然流產(chǎn)小鼠胎盤細胞凋亡及其相關調(diào)控基因FasFasL，Bcl2Bax的表達姓名：張列轉(zhuǎn)申請學位級別：碩士專業(yè)：微生物指導教師：米亞英20070510山西醫(yī)科大學碩士學位論文ApoptosisandexpressionofFas／FasLandBcl一2／BaxintrophoblastanddeciduaofspontaneousabortionmousemodelsAbstractobjective：Inor

2、dertoinvestigatethepathogeneticmiachnismofspontaneousabortionandtolookforavailabletherapetuticstheroy，weresearchapoptosisandexpressionofBcl2andBaxindeciduastissueofspontaneousabortionmousemodelsandnormalpregnancymousemod

3、elsrespectivelyMethods：TheabortionproneCBA／JXDBA／2matingswereestablishedasthemodelofspontaneousabortionandThenonabortionproneCBA／JXBALB／cmatingswereusedasthemodelofnormalpregnancySABCwasusedtodetecttheexpressionofBcl一2an

4、dBaxintrophoblastanddeciduatissueofspontaneousabortionandnormalpregnancymodelatday13ofgestationMedicalcolorifiaagemeasuresystemforimmune—histochemistry—WasusedforsemiquamitativeanalysisApoptosisWasdetectedbyusingDNAinsit

5、uterminaldeoxynuclotidyltransferasmediateddUTPbiotinnickendinglabeling(TUNEL)Results：(1)AIoftrophoblastanddeciduatissueinspontaneousabortionmodelwassignificantlyhigherthanthatofnormalpregnancymodel(戶O05)Theexpressionoffa

6、sLindeciduaofspontaneousabortionmodelwashigherthanthatofnormalpregnancymodel(P005)(3)TheexpressionofBaxinthetrophoblastofspontaneousabortionmodelwashigherthanthatofnormalpregnancymodel(Po05)：TheexpressionofBcl一2hadnosign

7、ificantdifferencebetweentwomodelinwophoblast(p005)TheexpressionofBaxinthedeciduaofspontaneousabortionmodelwashigherthanthatofnormalpregnancymodel(P005)，too；theexpressionofBcl一2waslowerthanthatofnormalpregnancymodel(P0，01

8、)’Conclusions：TobreaktheregulationofdecidualtissueapoptosismaydirectlyorindirectlyresultsinspontaneousaboaionduringearlygestationThechannelofBcl一2／BaxsystemsmaybeoneofthemechanismsofspontaneousabortiondeciduasapoptosisKe