中國部分綿羊群體的起源、遺傳多樣性及保護研究.pdf

1、中國農(nóng)業(yè)科學(xué)院博士學(xué)位論文中國部分綿羊群體的起源、遺傳多樣性及保護研究姓名：趙倩君申請學(xué)位級別：博士專業(yè)：動物遺傳育種與繁殖指導(dǎo)教師：馬月輝20070601AbstractToinvestigatetheorigingeneticslructm℃andgeneticrelationshipofChinesesheep，TansheepSunitesheep，Diqingsheep，Gangbasheep，L∞g蛐sheep，Yuxifa

2、ttailedsheep，InnerMongoliansemifinesheep，AIetysheep，Duolangsheep，F(xiàn)attailedbansheep，JinzhongsheepandmultihomedMongoliansheepwerestudiedbyusingmtDNAPolymorphismoffragmentofND5geneWeledetectedbyPCRSSCPoCompletesequenceofcen

3、nolregionandcythgeneweresequencedandNJureeandUPGMAtreeofshe卸、帆constl21ctedNetworkanalysisandpopulationexpansionwerecarriedout29microsatellitemarkerswere船lecmdtostudythegeneticvarianceof16ChinesesheeppopulationsHeterozygo

4、sitythenumberofallelescoefficientofgenedifferentiationgeneticdistancewerecalculatedThedendgramwereconstructedbasedongeneticdistanceMarginaldiversitymethodwasappliedtosetthepriorityofconservationBasedontheanalysisofextinc

5、tionprobabilitycontributionrateandconservationpotencytheoptimalfundallocationweremadeTheconclusionsWereasfollows：l，3haplutypegroupsweredetectedbyPCRsscPandhaplutypeAisdominantinChinesesheeppopulations2、Dloopsequencesofl1

6、4individualsfrom15populationsshared105haplotypeswhichwereclusteredinto4lineagesbasedOilphylogeneticanalysisLineageDwasanovellineageinthepresentstudyTheresuRsuggestedthatChinesesheepderivedfromthreeindependentmaternalance

7、storsatleastAnditsupponMofolonhadcloselyrelationshipwithChinesedomesticsheepNetworkanalysis，F(xiàn)ustestandmismatchdistributionimpliedthreelineagcs(A、Bandc、ofChinesesheepeverexperiencedpopulationexpansionpossibly3、Completecyt

8、hgeneof42individualsweresequencedandwhichshared40haplotypesThe4lineageswerefoundaccordingtophylogeneficanalysis4、Theresultshowedtherewerelargegeneticdistancebetweenmultihomedsheepandcommondomesticsheep5、Itshowedtherewere

9、abundantgeneticdiversityinChinesesheeppopulationsaccordingtoanalysisusingmircosatclliteDNAmarkersThemeanPICandheterzygosityof16populationswere0698and074respectively6，11leNJdendgrambasedonD^distanceshowedtherewere4groupsi

10、n16sheeppopulations，whichweregenerallyagreementwiththebreedinghistoryanddistribution7、Basedonconservationpotencytheconservationprioritiesofsheeppopulationswereasfollows：Lailzhoufattailedsheep，Yuxifattailedsheep，Langkazis

11、heep，Gangbasheep，DiqingsheepSunitesheep，fattailedsheep，Hanzhongsheep，Tansheep，Guideblackfursheep，Yuxifattailedsheep，DuolangsheepandTengchongsheep8、Weevaluatedtheeffectivenessofdifferentmodelstogainthemaximalexpecteddiver