As-,2-O-,3-磁性納米微球磁感應加熱治療肝癌的實驗研究.pdf

1、Y9Z弓91分類號：B23￡：塑；g壁皇理：!密級：扭蜜[二絲“石學校代碼：!Q2豎學號：Q!i342UDC東南大學碩士學位論文As：03磁性納米微球磁感應加熱治療肝癌的實驗研究研究生姓名：塞建導師姓名：毯塞生教授趣杰熬授申請學位級別醫(yī)掌砸學科專業(yè)名稱癌理堂皇癌堡生堡雯論文提交日期至Q始璽晝且Q皇目論文答辯日期呈鯉皇生曼且Q壁目學位授予單位蠢直塞堂學位授予日期至咝生且目答辯委員會主席煎熬塑熬援評閱人蓮叢麴熬援圃建星敦援2004年06月0

2、5日英文摘要AbstractResearchofMagneticNano—microspheresContainingAs203inC。ombinationwithMagneticFluidHyperthermiaonliverCancerGraduate：SONGJiangSupervisor：ProfZHANGDongsheng，PmfiZHENG1ieDepartmentofPathologyandPathophysiologyB

3、asicSchoolofMedicalScience，SoutheastUniversity，2l0009NanjingChina【ObjeetivelQToprepareandanalysewithmagneticnanomicrospheres(MNM)andmagneticnano—microspherescontainingAs203(MNMA)；②Toinvestigatethec”otoxicityofMNMandMNMA；

4、③ToinvestigatethehistotoxicityofMNMandMNMA；④ToinvestigatethetherapeuticeffectsofMNMandMNMAincombinationwithmagneticfluidhyperthermia(MFH)0nthexenograftlivercancerinnudemice【MaterialsandMethodsJ④MNMAwerepreparedbythetechn

5、iqueofchemicalcoprecipitationanditscharacteristicswereobservedbymeansoftransmissionelectronmicroscope(TEM)②ThecytotoxicityofMNMWeredetectedbymeansofhaemolysistest、micronucleustest、MTTtestTheeffectsofMNMandMNMAtreatedwith

6、radioffequencymagneticfieldonhepatocarcinomacellswerealsoevaluatedrespectively⑧ThemiceLD50ofMNMandMNMAwereevaluated，thenMNMandMNMAwereinjectedintotheliversofhealthypigstoobservethetheireffectsonnormalpigliver④Afterinject

7、edintoxenografthumanhepatocarcjnomaonnudemicerespectively，MNMandMNMAwereexposedtadiofrequencymagneticfieldforthreetimes15dayslater，theinhibitionratioofthetumorsandtheireffectsonexpressionoftumourmetastasisassociatedgenei

8、nperipheralbloodwereevaluated[Results】(!)ItwasconfirmedbyEDS(energydispersivespectrometer)thattheMNIvlandMNMAwereround，withanaveragediameterof20nmand1OOnmAtierexposedtoAMF，thetemperatureofthesuspensionofmagneticparticles

9、couldincreaseto41℃51℃correspondingtodifferentparticleconcentrations，andwouldkeepstablefromthenon②Itwasprovedbyhaemolysistest、micronueleustest、MTTtestthatMNMhasnocytotoxicityandMNMAcontainingdifferentconcentrationsofAs203

10、hasdifferentinhibitoryeffectsonfibroblastsandheptocarcinomacells③TheLD50ofbothMNIvlandMNMAtomicewerehigherthan59／kg1to4weeksafterinjectionofMNMandMNMAintohealthypigsliver，nosignificantdifferencesinseruluAST，ALT，BUNandCrl

11、evelsbetweenpigsofallgroupswerefound(p005)andnoobviouspathologicalalterationswereobserved④UndertheexposuretoAMF，theinhibitionratioofthetumorsofMNMgroupwere6874％，whichweresignificantlydifferentfromcontrolgroup(p0O】)；thein

12、hlbitionratioofthetulnorsofMNMAgroupwere8279％，whichwereverysignificantlydifferentfromcontrolgroup(pO001)Thetumorsofmiceintreatmentgroupshowedobviousnecrosis，whilethenormaltissueadjoiningthetumorandinternalorganswerenotda

13、magedpathologically⑨TheexpressionofCD44v6geneinPNMBCweredetectedbymeansofRT—PCRwhichshowedthattheirexpressioninmiceofAMF—exposuregroupwerelowerthannonAMFexposuregroup【ConclusionI(i3Withacontrollableandstabletemperature，M

14、NMandMNMAcouldexertradiofrequency—inducedhyperthermiaeffectontumors(勘AsMNMAcouldexertcombinedeffectsofdrugtoxicityandradiofrequency—inducedhyperthermiaontumorsanddonoharmtoliverorkidney，itisanidealcarrierfortumortargetin