轉基因抗蟲水稻對田間螟蟲結構的影響及其機理的初步研究.pdf

1、中國農業(yè)科學院碩士學位論文轉基因抗蟲水稻對田間螟蟲結構的影響及其機理的初步研究姓名：高玉林申請學位級別：碩士專業(yè)：農業(yè)昆蟲與害蟲防治指導教師：傅強20060601AbstlactEcolo舀caIsafbtyofn鋤sgellicricehasbeenbecommgafocusissue血tlleworldIt’sVeryiIllportantaIldnecessarytoevaluatet11epotentialec010百caIs疵

2、tysystematicallya11dscient訊callybefbretlecommercializationof仃aIlgenicriceInt11isp印er，tllee髓ctof訂ansgenicriceconta誠ng叼7朋c印口to)￡inonfieldcompositionofs廿ipedstemborer(ssB)，(確砌Jq即憎渤脅，arIdpmkstemborer(PSB)，&5冊f婦列備陀瑚were咖died，

3、鋤d沁possiblemechaIlismw越alsopreliminaIystudiedInaddjtion，thesusceptibiI時oft、vostemborerstoBtindividualorcombiIl撕onsw鵲alsostIldied，andthenhelptoguidetheselectionofefI毫ct～etoxinsforbreedingoftransgenicriceThemainresultswere

4、showedasfbllows1TheresuI協offieldtestsinbothearlyandlacericeindicatedtIatthetransgenlcnceproVidedhighlevelsofcontrolagaillstSSB鋤dPSBThenumberofSSB鋤dPSBlanr∞ontransgenicriceweresi印i矗ca|ltlyreducedcOmparedw汕thenon_仃鋤sgenicc

5、ontr01Amongthestemborerssurvivedont啪蹭enic—cefieId，mepercentageofPsBincre如eds咖incantlywhencomparedwiththatonnon一仃鋤sgeniccon仃D12ThelabOratorybio船sayresultsshowedthatthesuwivaIOfSSBarIdPSBontransgenicricewassiglli矗cantly10w

6、ert11aIlthatonnontran踞eniccontr01ThetoxicactiV時of打ansgenicricetoSSBandPSBdeclinedsign訊cantIyatthelategrowingstagePSBontraIlSgenjc訂cesurvivedobviouslybe釷erthanSSBAsforSSB，nolarvaecouldsurvivetothe5thdayafter_mfestationont

7、舢sgenjcriceAndsomePSBlarvae(2_3~73％)couldsurviveatthe15。dayFurthennore，therewereafewPSBlarvae(16％)couldpupa把andsomeofthemcouIdeme唱ence3Toxic時ofsevcralBtprotoxinsa鰣nsttwostemborers(PsBandsSB)wascompa他dbyfeedingneonate1aⅣa

8、eonaIti矗cialdietscontainingBtproto越nsiflthelabOraIolycOnditjonsTheresultsshowedsi舭incamsllSceptibil時di旋rencesbet、veenssBarIdPsBtocrylAb、crylAa、crylBa，withtheexceptionofthecrylcaandcrylAc，PsBwass培nmcalltlylowsusceptibleto

9、thefo盯nerthreeBtproto五ncompafedwithSSBTbevaluatepossjbleiⅡtemctionamongtheseprotoxjn，toxlci可assaywasperfbmledwithmixtIlresof2Btpmtoxinsat1：1ratioAlltIlemixnlreprotoxinshowedsigni矗cantsyne曙istictoxicityactivityagainstssBI

10、ncon訂髂t，onlyc叮1AacrylAbmixtureprotoxinindic砒edmoderatedsyne曙istictoxicityactiv姆againstPsBInmortality—timeresponseexperiments，PsBshowedmoretolerancetoCrylAbandC叫1ActhanSSBwhen訂eatedwjtllloworhighcOncentrationpmtoxjn4111ee

11、懦ctsofthetrallsgenic“ceonovipositiollalpre斯enceofPsB柚dthebiologyof4伊”，P伽加研P陽f珊，aparasitismfofPsB，wereinvest培atedItwaSobservedthatPsBdidnotobviouslyprefem砌tmsgenic—cetothecontrolriceinOvipositionalselection，a11dalso，thetr

12、ansgenicriceshowednosi印incante舵cton也ebiolo百calpar帥derofAg如肌Pmf塒species5Comp刪wjthPSBtreatedwilhthenon_trallsgenicrice，thev耐ationinbjologicaltransofsurvjvedPSBwasnotobservedwhentheywereselected(1～2generation)onn硼踞enicricei

13、nthefield，incIudjngpupalweight，emergencemIe，adultfecundilyandtheLC50toCrylAcTosumupIt’ssuggestedthat協eV耐edsusceptibil時oft、vostembo塒’totr鋤sgenicricefatherthaIltIleecologicalf她torpresenthere鏟eatlycon蜘butestoabe批funderstall