活性氧（ROS）對兔早期胚胎體外發(fā)育的影響.pdf

1、分類號UDCY9t0271密級學號延邊大學碩士學位論文活性氧(ROS)對兔早期胚胎體外發(fā)育的影響研究生姓名金慶國培養(yǎng)單位延邊大學農學院指導教師姓名、職稱方南洙教授學科專業(yè)研究方向論文提交日期動物遺傳育種與繁殖動物繁殖與生物技術2006年5月28日活性氧(ROS)對兔早期胚胎體外發(fā)育的妊響一AbmractEffectofreactiveoxygenspecies(ROS)onDevelopmentinEarlyStageofRabbitE

2、mbryosCulturedinvitroAbstractFSHhCGareappliedtabbitforsuperovulation，and2cellembryoscollectedfromoviductbysurgeryareculturedinvitroWeinvestigateintotheeffectofReactiveOxygenSpecies(ROS)H202ondevelopmentofrabbit2cellembry

3、oculturedinvitro，andsearchfortheROSsensitivephase，andprobeintotherelationshipbetweenROSanddevelopmentinearlystageofmammalianembryosculturedinvitroWecanoffertrustytheoreticalevidencesforincreasethedevelopmentrateofmammali

4、anembryosculturedinvitroandforembryologydevelopmentalbiologyandgeneticengineeringbyexperimentsTheblastularateofthecontrolgroups，三1189mol／Lgroups，1039mol／Lgroups，94pmol／Lgroups，and_88pmol／Lgroupsare86％，5％，26％，34％，and75％，r

5、espectivelyoNtheexperimentofthe2qellembryoswereculturedonTCM一199mediumwithdifferentH202levelsTherearesignificantlydifferencesbetween0～24hgroups(3714％)，24～48hgroups(5429％)andcontrolgroups(9142％)，andtherearenosignificantly

6、differencesbetween48～72hgroups(8286％)andcontrolgroups(9142％)ontheexperimentofdeterminewhichperiodhaveinhibitingeffectWeusefluorescencedensityteplaceH202and02一levelswithinembryo，becausethefluorescencedensityofDCFandEBarel

7、inearwithH202and02。levelsTheresultssuggestthattherearehigIlH202and02’levelsfrom4cellstagetomomlaTherearenosignificantlydifferencesbetween0～24hgroups(8226％)andcontrolgroups(9714％)，buttherearesignificantlydifferencesbetwee

8、n0～24hgroupsand24～48h(5250％)，48～72hgroups(30OO％)，moreovertherearesignificantlydifferencesbetween24～48hgroups(5250％1and4872hgroups(3000％1ontheexperimentoftheeffectofwithoutH202atdifferenttimeourabbitembryosculturedinvitro