dRASSF與Hippo的相互作用及其功能研究.pdf

1、南開大學(xué)碩士學(xué)位論文dRASSF與Hippo的相互作用及其功能研究姓名：李萍申請學(xué)位級別：碩士專業(yè)：遺傳學(xué)指導(dǎo)教師：吳世安201205AbstractAbstractTheHippopathwaywasfirstidentifiedasanovalorgansizecontrolsignalinginDrosophilabycoordinatelyregulatingcellgrowth，proliferationandapoptosi

2、sThecoreoftheHippopathwayconsistsofHippo，Warts，SalvadorandMatsHippobindstoSalvadortoformaHippoSalvadorkinasecomplex，whichthenactivatestheWarts—MatskinasecomplexWartsphosphorylatesYorkie，down—regulatesitstranscriptionacti

3、vation，andthendefinetheorgansizeAstheRaseffectors，RASSFproteinsspecificallybindtheRasviatheRAdomainRASSFproteinshavebeenimplicatedinarangeofbiologicalprocesses，includingtheregulationofcelldeath，cellcyclecontrolandmicrotu

4、bulestabilityandtheyaregenerallyregardedasturnoursuppressorsdRASSFtheDrosophilaorthologueofthehumanRASSFproteins，waspreviouslyreportedtestrictHippoactivitybycompetingwithSalvadorforbindingtoHippo，butthedetailedmechanismo

5、fthisfieldisstillnotclearInthisstudywefoundthatdRASSFinteractswithHippobutnotSalvadorandtheinteractionbetweendRASSFandHippoismediatedbytheSARAHprotein—proteinbindingdomainWeshowthatHippoandSalvadorlocateinthecytoplasm，an

6、ddRASSFlocatesinthenucleasmainlyWhenoverexpressionwithHippo，dRASSFtranslocatesintocytoplasmfromnucleasandcolocateswellwithHippointhecytoplasmToinvestigatethefunctionaleffectofthedRASSSFtoHippo，wecrossedtheUAS—hpo，nub—GAI

7、A／cyoflylinewithoverexpression，RNAiandknock—outflylinerespectivelyWefoundthatdRASSFCanprmotetheactivityofHippo，andthedifferentdomainsofdRASSFplaydifferentrolesInsummarythisstudydocummentsthatdRASSFprmotestheactivityofkin