轉(zhuǎn)基因技術(shù)改良水稻品種對(duì)病蟲(chóng)及除草劑抗性的研究.pdf

1、揚(yáng)州大學(xué)碩士學(xué)位論文轉(zhuǎn)基因技術(shù)改良水稻品種對(duì)病蟲(chóng)及除草劑抗性的研究姓名：王玲申請(qǐng)學(xué)位級(jí)別：碩士專業(yè)：作物遺傳育種指導(dǎo)教師：顧銘洪于恒秀20050601揚(yáng)州大學(xué)碩士學(xué)位論文Improvementoftheresistancetoinsect，diseaseandherbicideofricecultivarsbytransgenicapproachPostgraduate：WangLingSupervisors：ProfMinghongG

2、ufYangzhouUniversi“Yangzhou225009，JiangsuProvince)AbstractInordertoimprovetheresistancetodiseaseinsectandherbicideofricecultivatsbygeneticengineering，fourJaponicaricecultivars，Ouanglingxiangjing，Wuxiangjing9，4901，andWuxi

3、ang99—15，andtwolndicaricecultivars，Zhenshan97andXieqingzao，wereusedfortransformationwithfourtargetgenesmediatedbyAgrobacteriamThefourtargetgenesareBacillusthuringiensisfBt)cryIA(c)toxingene，theferredoxin—likeprotein(Apl)

4、geneencodinganamphipathicproteinfromsweetpepper，theGalanthusnivalisagglutinin(GNA，snowdroplectin)gene，andBargenefromStreptomyceshygroscopicus，whichhadbeenproventesistthestemborer，bacterialblightdisease，sap—suckinginsectp

5、ests，andBastaherbiciderespectively，afterexpressionintransgenicriceplantsByusingthetwinT—DNAscotransformationsystem，manyco—tranformedriceplantsweregeneratedineachtargetricevarietySubsequently，intheselfpollinedprogenyofco—

6、transformants，severalselectablemarkerfree(SMF)transgenicricelineswerescreened，andtheresistancetodiseasesandinsectsoftransgenmriceplantswerecarefullyanalyzedTheresuItswereshowedasfollowings：1Twotwin—T—DNAco—transformation

7、systems，ietwovectorsAwostrainsandsuperbinaryvector(twoseparateT—DNAregionsatonebinaryvect00，wereusedandtheirco—transformationefficiencyinricewerecomparedAlargenumberofco—transformantswereproduced，andtheresultsfrommolecul