兩株拮抗菌的發(fā)酵條件、定殖動態(tài)及生防效果研究.pdf

1、黑龍江八一農(nóng)墾大學碩士學位論文兩株拮抗菌的發(fā)酵條件、定殖動態(tài)及生防效果研究姓名：徐美娜申請學位級別：碩士專業(yè)：植物病理學指導(dǎo)教師：靳學慧王光華20050401Abs仃actSoilbomeDlamdiseasesiss州ousinchinawrhr弧ldI麗onbioIo西calco“旨olofF協(xié)a一硎r00tmtofsOybeaIl，whichisatyPicalone鋤ongsoiIbomeplantdise砸esMoren瑚10

2、如tagonisticis01種囂a印inStsoybe卸rotdi∞aSewerescre％ed矗DmsoybeanrhizosphefesoilT鉀oisol砒esieBRF1柚dBRF2haveS仃0119蛐dbToadspecnllmantagonisticab】時againstsevcnfhngalpathogensThet、vois01ateswcreident哺edbylllephysiolo百ca】柚dbiochemjc

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4、rialisolateswasinv哦i斟edbyllsmgthemut跚tswith柚tIbioticsrcsjstanceAfterBRF1鋤dBRF一2wasinocul舢edintotIlesoilmeire行bcl0nsoilmicrobialpopul撕oIlsanddivers時w豁d毗mmedbycultivationbascdmetllods，DGOE(den砷ⅡinggradientgeJele曲rophoresis

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7、矗ed船丑船講啦j曲f擊，byⅡlephysi010西cal鋤dbiochemicalcha蜥eri蚰csand16srRNAseqIlence3T1leexperim叻tal陀sllltsshow砸nlebestc徹d試0nsfbrBRF一1乎oⅥ脅鋤dantibioticsproducew鼬th砒ttlecllI_tummedi砌w舔PSswimpH7O，thevolurneof眥di啪was20IIlLin2somL砸列en如k粕

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10、i曲tempera伽re，strongacid柚dalkali5Eb，Il枷icCoIomza村0nTbgt：T11etwobiocontmlisDla把scouldcolonizeinso訂vervwellAfter也etlleyhadbeeninoc山atedfor48days，thederIs時ofBRFLandBRF一2respec吐velystlIlkeptover316103cFu，gsoil柚d6_3x104cFu幢soi

11、lBIu一1粕dBRF2couldinnuencedontlleq咖dtyandnlepolytf唧hismof“croo唱arIisminsoiLBdmofmetwois01atescouJdimDrovethelevelofsoil’sheallh6Therea陀900de丘托tofbiocOn仃olwh印weusedBRFl鋤dBRF2tocon扛DItllen船口，渤r00trotofsoybe柏，andtIleycouldim