馬鈴薯抗晚疫病體細胞無性系變異篩選技術(shù)研究.pdf

1、西北農(nóng)林科技大學(xué)碩士學(xué)位論文馬鈴薯抗晚疫病體細胞無性系變異篩選技術(shù)研究姓名：邢宇俊申請學(xué)位級別：碩士專業(yè)：蔬菜學(xué)指導(dǎo)教師：程智慧20040501StudyontheSelectionofCellMutantLinesResistanttoPhytophthorainfestansinpotatoPostgraduate:XingYujunSupervisor:Prof.ChengZhihui(CollegeofHorticultureN

2、orthwestScitechUniversityofAgricultureandForestryYanglingShaanxi712100China)AbstractTheresearchusedstemandleafofthreepotatocultivarFavoriteZaodabaiasexplantandtheculturefiltrateofPhytophthorainfestansasThebioactivitiesan

3、dcultureconditionofthecrudetoxinwereasDongnong303andtheselectiveagentstudy.ThenthecallusresistanttoPhytophthorainfestanswassayedatthebeginningofthemultistepscreeningmethodatdiferentconcentrationselectedbyusingonestepando

4、fthecrudetoxin.Thediseaseresistantmutantselectionsystemwasestablishedandseveralmutantplantswereobtained.withrye:crudetoxinwasobtunedbymeansofliquidcultureliquidmedium.Usingthecrudetoxinthestudy1Thecrudetoxinwasob面nedbPhy

5、tophthorainfestansontheleafandcallusofPhytophthorainfestansinvestigatedtheefectofofpotatothegerminationoftomatoseedsandthegrowthoftomatoradicel.Theresultsshowedthattheefectofcrudexplantswereconsistent.Thehighertheconcent

6、rationofetoxinonfourtypesoftoxicitywouldbe.Theefectsofcrudetoxincrudetoxinwasthestrongerthetomatoweremoredistinctthanonpotatotomatoseedscouldbeusedtostudythestudy.onseedgerminationandtheradicelgrowthofleafandcallus.There

7、forethegerminationrateofcultureconditionofcrudetoxininthefollowing2ThetoxinproductionofPhytophthorainfestanswasstudiedindiferentcultureconditionssuchasliquidmediumlighttemperaturepHvalueandshaking.Itwasappropriatefortoxi

8、nproductionthatpHvalueandtemperatureofculturemediumwere67and2022Crespectivelywithdarkeningandshaking24heverydayamong14and18dayintheryeliquidmedium.Ataboveconditionsthecrudetoxinshowedmoreinhibitiveefectsonseedgermination

9、oftomato3ThecrudetoxinofPhytophthorainfestanshadthecapacityofthermalstabilityandcouldnotonlyextremelyinhibitthegerminationoftomatoseedsbutalsodogreatharmtothegrowthradicel.4Crudetoxinastheselectedagentsomecellmutantlines

10、resistanttoPhytophthorainfestanswerescreenedbyleafstemandcallusfromtheminthreecultivarsbymeansofonestepandmultistepmethods.TheexperimentalresultsshowedtheresistanceofthreecultivarstoPhytophthorainfestanswasdiferent.Thehi

11、ghestresistantconcentrationtoPhytophthorainfestanscrudetoxinforFavoritewas400oandforDongnong303andZaodabaiwere50%.Withtheincreaseofthecrudetoxinconcentrationtherelativegrowthofcallusdecreasedandtheitscolourappearedbrown.

12、ThecallusfromstemwasmoreresistanttoPhytophthorainfestansthancallusfromleaf.Thegreatharmofcrudetoxintothegrowthofbudsdiferentiatedfromthecalluswasalsofound.5Theplantletsofthecontrolandfromthescreenedmutantwereculturedonme