28437.生物復合多色熒光共軛聚合物納米顆粒的制備及其在細胞靶向成像和檢測中的應用研究

1、分類號UDC中國科學院化學研究所博士后出站報告密級編號馮麗恒學科專業(yè)名稱：物堡絲堂工作結(jié)束時間：2Q!壘生!旦2014年1月當蘭ABSTRACTABSTRACTConjugatedpolymernanoparticles(CPNs)thatcontaintheexcellentoptoelectronicpropertiesofconjugatedpolymers(CPs)andthesizeeffectofnanoparticlesh

2、avebeensuccessfullypreparedandappliedintheinterdisciplinaryfieldsofmaterials，chemistrybiologyandmedicineTherapiddevelopmentofCPNsismainlyattributedtotheirsimplesynthesisproceduresandeasyseparationstepsTheadvantagesofCPNs

3、includehighbrightness，excellentphotostabilitylowcytotoxicityhighquantumyieldandlargemolarextinctioncoefficientInthework，basedonthecurrentbackgroundofCPNs，biofunctionalizedmulticolorCPNswerepreparedandappliedtoimageanddet

4、ectforrumorcellsTheresultsareasfollows：1Conjugatedpolymersthatcoverthefluorescenceemissionsfrombluetedweresynthesizedbyfluorenemonomer(donor)andaromaticheterocyclicmonomers(receptors)InordertoincreasesolubilityofCPtheCPs

5、weremodifiedbyalkyloralkanesoxygengroupsinsidechain2Throughthenano—precipitationmethod，CPNswithcarboxylgroupsinthesurfacewereobtainedTheCPNswiththewholevisiblelightemissionwererealizedbyregulatingthefluorescenceresonance

6、energyt啪sfer(FRET)processesofdifferentCPsBiofunctionalized(antibody)CPNswerepreparedbycondensationreactionofcarboxylgroupsofCPNsandaminogroupsofantibodies3TheimagingprocessesofbiofunctionalizedCPNstotumorcellswerestudied

7、bythefluorescenceimagingtechniqueTheresnltsshowthatthebiofunctionalizedCPNsCanimageandrecognizethetumorcellswithspecificityUsingsignalamplificationeffectofsecondaryantibody(IgG)toprimarymodifiedCPNs，thesensitivityofCPNst