29612.一株中度嗜鹽菌的分離、鑒定及系統(tǒng)發(fā)育分析

1、東北師范大學碩士學位論文一株中度嗜鹽菌的分離、鑒定及系統(tǒng)發(fā)育分析姓名：王柏玲申請學位級別：碩士專業(yè)：微生物學指導教師：劉東波20090601AbstractBytheclassicculture、methodandgradientdilutionisolationmethod，thestrainJLT01whichCallgrowoptimallyinculturemediathatcontain1015％NaClWasisolated

2、fromtheJilantaisaltlakelocatedinInnerMongoliaAccordingtotheclassicphenotypiccharacteristicswhichincludemorphologicalobservation、GrowthFactorandthephysiologicalandbio—chemicalcharacteristics，homologousalignmentandgradient

3、dilutioncultureinclude16SrDNAanalysis，(CrC)contentandtheDNADNAhybridization，themoderatehalophilestrainWasidentifiedtobelongtoMarinobacterItisGramnegative，non—sporeforming，noncapsule—forming，nonflagellum—forming，rodshaped

4、Coloniesarecream—coloured，smooth，circulartoslightlyregularOptimalgrowthOCCURSinthepresenceof10—12％(w／v)totalsalt，inthepresenceof3％(w／v)Mg，at370C，atpH75；Thefollowingcompoundsareutilizedassolecarbonandenergysources：glucose

5、，sucrose，galactose，Thefollowingcompoundsarenotutilizedassolecarbonandenergysources：fructose，maltose，ribose，sorbitol，rhamnose，arabinose，mannitol，mannose，xylose、lactoseMethylredandVogesProskauerarenegativeIndoleandH2Sarepo

6、sitiveSimmons’citratetestisposffiveNitrateandnitritearenotreducedCatalaseandoxidaseareproduced，Tween80ishydrolysed，butcasein、amylase，ureasearenotBenzidinehydrochlorideispositive，GelatinliquefactionisnegativeTodetermineth

7、etaxonomypositionofthenewlyisolatedmoderatehalophilebacteriumJLTO1inevolutionhistory16SrRNAsequencehomologousalignmentandphylogenyanalysiswereperformedbetweenitandrelatedspeciesTheDNA—DNAhybridizationresultsshowedthatthe

8、strainWasnotrelatedatspeciesleveltotheMarinobactertipotyticusBasedonthedataofpolyphasictaxonomyweproposetoassignstrainJLT01toanovlespecieofthegenusMarinobacter，forwhichweproposethenameMarinobacterJilantaisumspnovThetypes