30542.三類轉(zhuǎn)錄因子家族在葡萄基因組中的分析及兩個辣椒果實(shí)成熟相關(guān)轉(zhuǎn)錄因子的研究

1、重慶大學(xué)博士學(xué)位論文三類轉(zhuǎn)錄因子家族在葡萄基因組中的分析及兩個辣椒果實(shí)成熟相關(guān)轉(zhuǎn)錄因子的研究姓名：王翊申請學(xué)位級別：博士專業(yè)：生物工程指導(dǎo)教師：陳國平20090614英文摘要ABSTRACTTranscriptionfactorsareimportantregulatorstoactivateorrepresstheexpressionofcodingornon—codinggenes，throu曲whichtheycanfurther

2、influenceorcontrolmanybiologicalprocessesInplants，asinalllivingorganisms，transcriptionfactorsareanimportantlevelofgeneregulationManyfamiliesoftranscriptionfactorshavebeenidentifiedinplantsAnalysisoftheArabidopsisgenomere

3、vealsmanytranscriptionfactorfamilies，atleast16ofwhichappeartobeuniquetoplantsInthesefamilies，MADS—boxandHomeoboxgenesformtwolargegenefamiliesoftranscriptionfactorsthatcontrolfundamentalprocessesofplantdevelopmentInadditi

4、onSBPboxgenefamilybelongstoagroupofplantspecificzincfingerproteingenesthatencodeplant—specifictranscriptionfactorsInrecentyears，somereportsshowthatHomeobox，MADSandSBParerelatedinberryfruitripeningInthispaperusingESTdataw

5、ereportanumberofgenes，especiallysometranscriptionfactorsthatpossiblyareinvolvedintomatofruitdevelopmentTheresulthasbeentheopeningofanewfrontierinripeningmolecularbiologythatisfocusedonupstreamtranscriptionalcontr01Theres

6、earchofthesetranscriptionfactorsinvolvedberryfruitripeningisonlygetfromtomato，fewstudiesareperformedinotherberryspecies，suchasgrapeandpepperInthispaperagenome—widecharacterizationofthethreetranscriptionfamilieshasbeencon

7、ductedinthegrapevineHere，weprovideacompleteoverviewofthesefamilies，describingthegenestructure，geneexpressiongenomelocalizationproteinmotiforganizationandphylogeneticrelationshipofeachmemberThecomparisonofthethreetranscri

8、ptionfactorfamiliesinhomologandexpressionpattemsbetweengrapeandtomatosuggestedthatthesetranscriptionfatorsingrapemightplayanimportantroleintheregulationoffruitripeningThecomprehensiveannotationinthesetranscriptionfactors

9、investigationsaddstoourunderstandingandidentifiescandidategenesforfuturefunctionalvalidationstudiesInaddition，weisolatedandcharacterizedtwonoveltranscriptionfactorsCaRlNandCaSPLfromthechilipepperCapsicumannuumLcvBukangSe